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. 2022 Sep 8:10:1014208.
doi: 10.3389/fchem.2022.1014208. eCollection 2022.

OEA loaded liposomes with the neuroprotective effect for stroke therapy

Shichao Wu  1   2   3 , Xiangrui Yang  1   2   3
Affiliations

OEA loaded liposomes with the neuroprotective effect for stroke therapy

Shichao Wu et al. Front Chem. .

Abstract

With high mortality, stroke has become a serious threat to human health. Nevertheless, the strategy for stroke therapy is quite limited in the clinic till now. In this research, we prepared a novel neuroprotective nanoformulation (OEA Liposomes) via encapsulating endogenous N-oleoylethanolamine (OEA) in liposomes for intravenous administration. The formulation largely increased the solubility and bioavailability of OEA. Then the following systematic experiments stated the excellent neuroprotective effect of OEA Liposomes in vivo. The survival rate of the nanodrug group was largely increased to 75%, while that of the Middle Cerebral Artery Occlusion (MCAO) group was only 41.7%. And the severe neurological functional deficit of the MCAO rats was also significantly improved. What's more, the OEA Liposomes could inhibit the apoptosis of neurons and the inflammation of reperfusion to a very slight level, indicating their outstanding neuroprotective effect. These results indicated that the OEA Liposomes have a great potential for clinic anti-stroke application.

Keywords: OEA; drug delivery; ischemia reperfusion; neurprotective effect; stroke.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
The TEM images of OEA Liposomes.
FIGURE 2
FIGURE 2
The size (A) and in vitro drug release profiles (B) of OEA Liposomes.
FIGURE 3
FIGURE 3
The survival rate (A) and the Garcia scores (B) of the MCAO rats administrated with the indicated formulations.
FIGURE 4
FIGURE 4
Representative pictures of the cortex stained with TUNEL.
FIGURE 5
FIGURE 5
The statistical data (D) of the cortex stained with TUNEL. Data are expressed as mean ± SEM. *** = p < 0.001, compared to MCAO group).
FIGURE 6
FIGURE 6
(A) The area of IBA-1 and GFAP positive stain from the cortex around the ischemic focus. (B–C) The enlarged pictures of IBA-1 and GFAP positive area.
FIGURE 7
FIGURE 7
The statistical data of IBA-1 (A) and GFAP (B) positive stain from the cortex around the ischemic focus. Data are expressed as mean ± SEM. (**** = p < 0.0001, compared to MCAO group).
See this image and copyright information in PMC

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