Discovery of the First Lytic Staphylococcus pseudintermedius/ Staphylococcus aureus Polyvalent Bacteriophages

doi:10.1089/phage.2022.0004

. 2022 Jun 1;3(2):116-124.

doi: 10.1089/phage.2022.0004. Epub 2022 Jun 16.

Discovery of the First Lytic Staphylococcus pseudintermedius/ Staphylococcus aureus Polyvalent Bacteriophages

Haroldo Hernandez Santos^{1

2}, Justin Clark^{1

2}, Austen Terwilliger^{1

2}, Anthony Maresso^{1

2}

Affiliations

¹ Tailored Antibacterials and Innovative Laboratories for Phage (Φ) Research (TAILΦR), Baylor College of Medicine, Houston, Texas, USA.
² Molecular Virology and Microbiology Department, Baylor College of Medicine, Houston, Texas, USA.

PMID: 36157281
PMCID: PMC9248872
DOI: 10.1089/phage.2022.0004

Discovery of the First Lytic Staphylococcus pseudintermedius/ Staphylococcus aureus Polyvalent Bacteriophages

Haroldo Hernandez Santos et al. Phage (New Rochelle). 2022.

. 2022 Jun 1;3(2):116-124.

doi: 10.1089/phage.2022.0004. Epub 2022 Jun 16.

Authors

Haroldo Hernandez Santos^{1

2}, Justin Clark^{1

2}, Austen Terwilliger^{1

2}, Anthony Maresso^{1

2}

Affiliations

¹ Tailored Antibacterials and Innovative Laboratories for Phage (Φ) Research (TAILΦR), Baylor College of Medicine, Houston, Texas, USA.
² Molecular Virology and Microbiology Department, Baylor College of Medicine, Houston, Texas, USA.

PMID: 36157281
PMCID: PMC9248872
DOI: 10.1089/phage.2022.0004

Abstract

Background: There are no verified lytic Staphylococcus pseudintermedius phages in the literature and few temperate phage genomes in databases. S. pseudintermedius is an opportunistic zoonotic pathogen of great importance in veterinary and human medicine.

Materials and methods: We discovered phages against canine-derived S. pseudintermedius isolates by screening dog feces, hair, and skin swabs. Fourteen new phages were isolated and characterized by genomic analysis, transmission electron microscopy, and host range determination.

Results: Three phages-DH2, DH5, and DS10, a phage K variant-were predicted lytic by sequencing, a designation supported by mitomycin C induction. All three are S. pseudintermedius and Staphylococcus aureus polyvalent phages, with DH2 and DS10 being strong killers of both species.

Conclusions: We report discovery of the first verified lytic S. pseudintermedius phages and suggest dog hair as a novel reservoir. DH2, DH5, and DS10 are promising candidates toward developing an anti-Staphylococcal phage cocktail.

Keywords: canine; lytic; pseudintermedius; pyoderma; virulent.

PubMed Disclaimer

Conflict of interest statement

No competing financial interests exist.

Figures

**FIG. 1.**
Bacterial and phage isolation from random canine samples. Pellets were streaked onto mannitol salt agar plates, with isolates sequenced based on catalase positivity. Identified *Staphylococcal* and Gram-positive isolates are shown. A phage hunt against *Staphylococcus aureus* and *Staphylococcus pseudintermedius* isolates was performed using a double agar overlay spot assay with the concentrated filtrates. This figure was created with BioRender.com

**FIG. 2.**
Morphology and host range of predicted lytic *Staphylococcus pseudintermedius* phages. **(A)** Top: Phages imaged by transmission electron microscopy. Bottom: Plaque size and morphology on strains AZM1, AZM37, and TCH1516, respectively. Each tick represents 1 mm. **(B)** Host range determined through efficiency of plating on 10 *Staphylococcus. aureus* and 15 *S. pseudintermedius* strains. This figure was created with BioRender.com

See this image and copyright information in PMC

Cited by

Antimicrobial Strategies Proposed for the Treatment of S. pseudintermedius and Other Dermato-Pathogenic Staphylococcus spp. in Companion Animals: A Narrative Review.
Stefanetti V, Passamonti F, Rampacci E. Stefanetti V, et al. Vet Sci. 2024 Jul 11;11(7):311. doi: 10.3390/vetsci11070311. Vet Sci. 2024. PMID: 39057995 Free PMC article. Review.
Topical Bacteriophage Therapy for Staphylococcal Superficial Pyoderma in Horses: A Double-Blind, Placebo-Controlled Pilot Study.
Marshall K, Marsella R. Marshall K, et al. Pathogens. 2023 Jun 14;12(6):828. doi: 10.3390/pathogens12060828. Pathogens. 2023. PMID: 37375518 Free PMC article.
Methicillin-resistant Staphylococcus pseudintermedius: epidemiological changes, antibiotic resistance, and alternative therapeutic strategies.
Nocera FP, De Martino L. Nocera FP, et al. Vet Res Commun. 2024 Dec;48(6):3505-3515. doi: 10.1007/s11259-024-10508-8. Epub 2024 Aug 21. Vet Res Commun. 2024. PMID: 39167258 Free PMC article. Review.
Successful phage-antibiotic therapy of P. aeruginosa implant-associated infection in a Siamese cat.
Braunstein R, Hubanic G, Yerushalmy O, Oren-Alkalay S, Rimon A, Coppenhagen-Glazer S, Niv O, Marom H, Barsheshet A, Hazan R. Braunstein R, et al. Vet Q. 2024 Dec;44(1):1-9. doi: 10.1080/01652176.2024.2350661. Epub 2024 May 10. Vet Q. 2024. PMID: 38726795 Free PMC article.

References

1. Devriese LA, Vancanneyt M, Baele M, et al. Staphylococcus pseudintermedius Sp. Nov., a coagulase-positive species from animals. Int J Syst Evol Microbiol 2005;55(4):1569–1573; doi: 10.1099/ijs.0.63413-0 - DOI - PubMed
1. Ross Fitzgerald J. The Staphylococcus intermedius group of bacterial pathogens: Species re-classification, pathogenesis and the emergence of meticillin resistance: Recent studies of the staphylococcus intermedius group. Vet Dermatol 2009;20(5–6):490–495; doi: 10.1111/j.1365-3164.2009.00828.x - DOI - PubMed
1. Weese JS, van Duijkeren E. Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Vet Microbiol 2010;140(3–4):418–429; doi: 10.1016/j.vetmic.2009.01.039 - DOI - PubMed
1. van Duijkeren E, Catry B, Greko C, et al. Review on methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother 2011;66(12):2705–2714; doi: 10.1093/jac/dkr367 - DOI - PubMed
1. Lynch SA, Helbig KJ. The complex diseases of Staphylococcus pseudintermedius in canines: Where to next? Vet Sci 2021;8(1):11; doi: 10.3390/vetsci8010011 - DOI - PMC - PubMed

LinkOut - more resources

Full Text Sources
- Europe PubMed Central
- PubMed Central

[1] Devriese LA, Vancanneyt M, Baele M, et al. Staphylococcus pseudintermedius Sp. Nov., a coagulase-positive species from animals. Int J Syst Evol Microbiol 2005;55(4):1569–1573; doi: 10.1099/ijs.0.63413-0 - DOI - PubMed

[2] Devriese LA, Vancanneyt M, Baele M, et al. Staphylococcus pseudintermedius Sp. Nov., a coagulase-positive species from animals. Int J Syst Evol Microbiol 2005;55(4):1569–1573; doi: 10.1099/ijs.0.63413-0 - DOI - PubMed

[3] Ross Fitzgerald J. The Staphylococcus intermedius group of bacterial pathogens: Species re-classification, pathogenesis and the emergence of meticillin resistance: Recent studies of the staphylococcus intermedius group. Vet Dermatol 2009;20(5–6):490–495; doi: 10.1111/j.1365-3164.2009.00828.x - DOI - PubMed

[4] Ross Fitzgerald J. The Staphylococcus intermedius group of bacterial pathogens: Species re-classification, pathogenesis and the emergence of meticillin resistance: Recent studies of the staphylococcus intermedius group. Vet Dermatol 2009;20(5–6):490–495; doi: 10.1111/j.1365-3164.2009.00828.x - DOI - PubMed

[5] Weese JS, van Duijkeren E. Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Vet Microbiol 2010;140(3–4):418–429; doi: 10.1016/j.vetmic.2009.01.039 - DOI - PubMed

[6] Weese JS, van Duijkeren E. Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Vet Microbiol 2010;140(3–4):418–429; doi: 10.1016/j.vetmic.2009.01.039 - DOI - PubMed

[7] van Duijkeren E, Catry B, Greko C, et al. Review on methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother 2011;66(12):2705–2714; doi: 10.1093/jac/dkr367 - DOI - PubMed

[8] van Duijkeren E, Catry B, Greko C, et al. Review on methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother 2011;66(12):2705–2714; doi: 10.1093/jac/dkr367 - DOI - PubMed

[9] Lynch SA, Helbig KJ. The complex diseases of Staphylococcus pseudintermedius in canines: Where to next? Vet Sci 2021;8(1):11; doi: 10.3390/vetsci8010011 - DOI - PMC - PubMed

[10] Lynch SA, Helbig KJ. The complex diseases of Staphylococcus pseudintermedius in canines: Where to next? Vet Sci 2021;8(1):11; doi: 10.3390/vetsci8010011 - DOI - PMC - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Discovery of the First Lytic Staphylococcus pseudintermedius/ Staphylococcus aureus Polyvalent Bacteriophages

Affiliations

Discovery of the First Lytic Staphylococcus pseudintermedius/ Staphylococcus aureus Polyvalent Bacteriophages

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

Similar articles

Cited by

References

LinkOut - more resources

Full Text Sources