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. 2022 Sep 16:2022:4743041.
doi: 10.1155/2022/4743041. eCollection 2022.

Antiulcerogenic and Antibacterial Effects of Chitosan Derivatives on Experimental Gastric Ulcers in Rats

Leudimar Aires Pereira  1 , Luizângela da Silva Reis  2 , Anderson Nogueira Mendes  3 , Hélio de Barros Fernandes  3 , Daniel Dias Rufino Arcanjo  3 , Adalberto Fortes Rodrigues Júnior  4 , Janete Martins Sousa  1 , Humberto Medeiros Barreto  5 , Jailson de Araújo Santos  1 , Josy Anteveli Osajima  1 , Edson C Silva Filho  1
Affiliations

Antiulcerogenic and Antibacterial Effects of Chitosan Derivatives on Experimental Gastric Ulcers in Rats

Leudimar Aires Pereira et al. Evid Based Complement Alternat Med. .

Abstract

Gastric ulcer is an injury that develops on the lining of the stomach due to an imbalance between aggressive and defensive agents. Chitosan derivatives demonstrate promising biological activities in accelerating the healing activity of gastric lesions. Thus, this study aimed at investigating the healing activity of gastric lesion, induced by acetic acid (80%), of the chitosan derivative with acetylacetone (Cac) modified with ethylenediamine (Cacen) or diethylenetriamine (Cacdien). The biological activity was determined based on cytotoxicity, antibacterial activity, and gastroprotective activities. The results showed no significant difference in the cytotoxicity, a better antibacterial activity against S. aureus and E. coli, and a positive result on the healing of gastric lesions of the materials (Cac 18.4%, Cacen 55.2%, and Cacdien 68.1%) compared to pure chitosan (50.7%). Therefore, the results indicate that derivatives of chitosan are promising biomaterials for application in the control of lesions on the gastric mucosa.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Effect of chitosan and its derivatives on the viability of GM07492A (human fibroblasts). Cell viability was assessed by the MTT assay after 24 h incubation with chitosan (C): chitosan modified with acetylacetone (Cac), chitosan modified with ethylenediamine (Cacen), and chitosan modified with diethylene triamine. Data are presented as mean ± SEM, obtained from three independent experiments (n = 3) in triplicate. The statistical significance for the differences among the groups was calculated using analysis of variance (ANOVA) and Tukey's post hoc test. Differences between the groups were considered significant when p < 0.05.
Figure 2
Figure 2
Inhibitory effect of chitosan and its derivatives on the growth of S. aureus and E coli. The inhibitory effect was evaluated after 48 h and 72 h of incubation of the bacteria with chitosan (C): acetylacetone-modified chitosan (Cac), ethylenediamine-modified chitosan (Cacen), and diethylenetriamine-modified chitosan. Data are presented as mean ± SEM, obtained from three independent experiments (n = 3) in triplicate.
Figure 3
Figure 3
Effect of the gastroprotective activity of chitosan and its derivatives. Effect of chitosan gastroprotective activity (C): acetylacetone-modified chitosan (Cac), ethylenediamine-modified chitosan (Cacen), and diethylenetriamine-modified chitosan, cimetidine (100 mg/kg), after induction of gastric injury with acetic acid. (a) Area of residual lesion after treatment with chitosan and its derivatives. (b) Percentage of area recovered after gastric injury and treatment. Data are presented as mean ± SEM, obtained from three independent experiments (n = 3) in triplicate. ∗p > 0.5.
Figure 4
Figure 4
Gastric ulcer induced by acetic acid in rats treated, orally, for seven days with vehicle/injured control (a), cimetidine (100 mg/kg) (b), daily dose of 80 mg/kg of chitosan (c) and derivatives Cac (d), Cacen (e), and Cacdien (f).
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