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Comment
. 2022 Sep 19;2(9):100295.
doi: 10.1016/j.crmeth.2022.100295.

A new system for multiplexed mosaic analysis of gene function in the mouse

Hongchen Cai  1 , Monte M Winslow  1   2
Affiliations
Comment

A new system for multiplexed mosaic analysis of gene function in the mouse

Hongchen Cai et al. Cell Rep Methods. .

Abstract

In a recent issue of Cell, Liu et al. present an innovative mouse model system in which Cre/lox stochastically turns on transgenic expression of one out of up to 100 sgRNAs in somatic cells, creating genetic mosaicism that enables the multiplexed assessment of gene function in vivo.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Cre/lox-regulated sgRNA expression to create genetic mosaics in vivo Liu et al. (2022) developed iMAP in which CreER recombines the upstream Lox71 and one of the many LoxTC9 sites, resulting in a mosaic of cells expressing different sgRNAs across various tissues and organs. The highlights of this mouse model system and potential future applications are indicated.
See this image and copyright information in PMC

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