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. 2022 Sep 9:13:1006940.
doi: 10.3389/fpls.2022.1006940. eCollection 2022.

Silencing of Sly-miR171d increased the expression of GRAS24 and enhanced postharvest chilling tolerance of tomato fruit

Zengting Xing  1 , Taishan Huang  1 , Keyan Zhao  1 , Lanhuan Meng  1 , Hongmiao Song  1 , Zhengke Zhang  1 , Xiangbin Xu  1 , Songbai Liu  1   2
Affiliations

Silencing of Sly-miR171d increased the expression of GRAS24 and enhanced postharvest chilling tolerance of tomato fruit

Zengting Xing et al. Front Plant Sci. .

Abstract

The role of Sly-miR171d on tomato fruit chilling injury (CI) was investigated. The results showed that silencing the endogenous Sly-miR171d effectively delayed the increase of CI and electrolyte leakage (EL) in tomato fruit, and maintained fruit firmness and quality. After low temperature storage, the expression of target gene GRAS24 increased in STTM-miR171d tomato fruit, the level of GA3 anabolism and the expression of CBF1, an important regulator of cold resistance, both increased in STTM-miR171d tomato fruit, indicated that silencing the Sly-miR171d can improve the resistance ability of postharvest tomato fruit to chilling tolerance.

Keywords: GA; GRAS; Sly-miR171d; chilling tolerance; tomato.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Surface CI symptoms and CI index of red ripe tomato fruit. (A) Symptoms of CI on the surface of tomato fruit. (B) CI index. The error bar represents the independent repeat SDs (n = 3). The asterisk indicates that the data difference with P < 0.05 is statistically significant.
FIGURE 2
FIGURE 2
Surface CI symptoms and CI index of green ripe tomato fruit. (A) Symptoms of CI on the surface of tomato fruit. (B,C) CI index. The error bar represents the independent repeat SDs (n = 3). The asterisk indicates that the data difference with P < 0.05 is statistically significant.
FIGURE 3
FIGURE 3
Electrolyte leakage, Firmness, TSS, and GA3 content of tomato fruit in red ripening stage (A) and green ripening stage (B). The error bar represents the independent repeat SDs (n = 3). The asterisk indicates that the data difference with P < 0.05 is statistically significant.
FIGURE 4
FIGURE 4
The expression levels of CBF1 and COR genes in red ripe tomato fruit (A) and green ripe tomato fruit (B). The error bar represents the independent repeat SDs (n = 3). The asterisk indicates that the data difference with P < 0.05 is statistically significant.
FIGURE 5
FIGURE 5
The expression levels of Sly-miR171d and SlGRAS24 genes in red ripe tomato fruit (A) and green ripe tomato fruit (B). The error bar represents the independent repeat SDs (n = 3). The asterisk indicates that the data difference with P < 0.05 is statistically significant.
FIGURE 6
FIGURE 6
The expression levels of GA20ox1, GA3ox1, GA2ox1, and GAI genes in red ripe tomato fruit (A) and green ripe tomato fruit (B). The error bar represents the independent repeat SDs (n = 3). The asterisk indicates that the data difference with P < 0.05 is statistically significant.
FIGURE 7
FIGURE 7
A simplified model of the regulatory mechanism of Sly-miR171d on cold tolerance of tomato fruit stored at low temperature.
See this image and copyright information in PMC

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