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. 2022 Feb;52(1):268-275.
doi: 10.3906/sag-1911-14. Epub 2022 Feb 22.

A high-fat and high-fructose diet lowers the cecal digesta's weight and short-chain fatty acid level of a Sprague-Dawley rat model

Etik Sulistyowati  1 , Dian Handayani  2 , Setyawati Soeharto  3 , Achmad Rudijanto  4
Affiliations

A high-fat and high-fructose diet lowers the cecal digesta's weight and short-chain fatty acid level of a Sprague-Dawley rat model

Etik Sulistyowati et al. Turk J Med Sci. 2022 Feb.

Abstract

Background: This study aimed to analyze the effect of a high-fat and high-fructose diet (HFFD) on the digesta weight and shortchain fatty acid (SCFA) levels of cecal digesta in rats.

Methods: This study was an experimental study with a posttest-only control group design with male Sprague-Dawley strain rats as the samples. A total of 36 rats were divided into two groups with normal diet (N) and modified HFFD. The data of energy intake, nutrients and fiber, body weight, Lee index, abdominal circumference, digesta weight, and SCFA levels of cecal digesta were collected.

Results: The results showed an 11.94% increase in body weights of rats with HFFD. The total energy intake of the HFFD group was significantly higher than that of N (p = 0.000). The fiber intake and cecal digesta weight in group N were higher than that in the HFFD group (p = 0.00 and p = 0.02, respectively). The concentrations of acetate, butyrate, propionate, and total SCFA in the N group were significantly higher than in the HFFD (p = 0.041,,p = 0.004, p = 0.040, p = 0.013, respectively). A significant negative relationship was observed between the abdominal circumference and cecal digesta concentration (p = 0.029; r = -0.529) and between the Lee index and the SCFA concentration of cecal digesta (p = 0.036, r = -0.206).

Keywords: Cecal digesta; SCFA; diet; high-fat; high-fructose; short-chain fatty acid.

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Conflict of interest statement

Conflict of interest

There are no conflicts of interest.

Figures

Figure
Figure
The average weight of cecal digesta by group.
See this image and copyright information in PMC

References

    1. Greenwood HC, Bloom SR, Murphy KG. Peptides and their potential role in the treatment of diabetes and obesity. The Review of Diabetic Studies: RDS. 2011;8(3):355–368. doi: 10.1900/RDS.2011.8.355. - DOI - PMC - PubMed
    1. Shrimpton R, Rokx C. Health, Nutrition and Population Discussion Paper. Washington, DC, USA: World Bank Group; 2012. The DOUBLE BURDEN OF MALNUTRITION: A REVIEW OF GLOBAL EVIDENCE.
    1. Forse RA, Krishnamurty DM. Epidemiology and discrimination in obesity. In: Nguyen NT, Blackstone RP, Morton JM, Ponce J, Rosenthal RJ, editors. The ASMBS Textbook of Bariatric Surgery. Vol. 1. New York, NY, USA: Springer; 2015.
    1. Ye J. Mechanisms of insulin resistance in obesity. Frontiers of Medicine. 2013;7(1):14–24. doi: 10.1007/s11684-013-0262-6. - DOI - PMC - PubMed
    1. Lavie CJ, De Schutter A, Patel DA, Romero-Corral A, Artham SM, et al. Body composition and survival in stable coronary heart disease: impact of lean mass index and body fat in the “obesity paradox”. Journal of the American College of Cardiology. 2012;60(15):1374–80. doi: 10.1016/j.jacc.2012.05.037. - DOI - PubMed

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