Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Oct;136(1):191-200.
doi: 10.1128/jb.136.1.191-200.1978.

Purification and characterization of a tRNA methylase from Salmonella typhimurium

W T Pope, R H Reeves

Purification and characterization of a tRNA methylase from Salmonella typhimurium

W T Pope et al. J Bacteriol. 1978 Oct.

Abstract

A tRNA methylase, in which supK strains of Salmonella typhimurium are deficient, was purified from strain LT2 and characterized. Column chromatography of protein extracts from wild-type cells on phosphocellulose, diethylaminoethyl-Sephadex A-50, and hydroxlapatite resulted in an enzyme that was estimated to be about 50% pure. tRNA from S. typhimurium which had been incubated at pH 9.0 served as a substrate for this methylase. The enzyme has a molecular weight of about 50,000 as estimated by gel chromatography and by electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. The optimal assay conditions, as well as the kinetics and stability of the enzyme, were studied. As with other tRNA-methylating enzymes, S-adenosylhomocysteine is a potent inhibitor.

PubMed Disclaimer

References

    1. J Biol Chem. 1956 Jan;218(1):97-106 - PubMed
    1. J Biol Chem. 1964 Oct;239:3474-82 - PubMed
    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Nucleic Acids Res. 1978 Mar;5(3):1041-57 - PubMed

Publication types

MeSH terms