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. 2022 Oct 19;60(10):e0108422.
doi: 10.1128/jcm.01084-22. Epub 2022 Sep 28.

Vibrio cholerae Isolation from Frozen Vomitus and Stool Samples

Chelsea N Dunmire  1 , Denise Chac  1 , Fahima Chowdhury  2 , Ashraful I Khan  2 , Taufiqur R Bhuiyan  2 , Regina C LaRocque  3 , Afroza Akter  2 , Mohammad Ashraful Amin  2 , Edward T Ryan  3 , Firdausi Qadri  2 , Ana A Weil  1
Affiliations

Vibrio cholerae Isolation from Frozen Vomitus and Stool Samples

Chelsea N Dunmire et al. J Clin Microbiol. .
No abstract available

Keywords: Vibrio cholerae; isolation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Vibrio cholerae (Vc) isolation and pH from a subset of frozen samples and the relationship between these factors and CFU count from the fresh sample; n = 18 for each sample type. Vc CFU were enumerated from fresh plating of stool selective tellurite taurocholate gelatin agar. (A) Vc isolation of vomitus and (B) stool samples. Each data point represents one sample. “Isolated from sample without preservative” indicates that Vc was isolated from samples stored in no preservative. If this method of isolation failed, samples stored in glycerol were attempted, and successful where “Isolation from glycerol-stored sample only” is indicated. If both glycerol-preserved samples and no preservative samples failed Vc isolation, “No Vc recovery” is shown. Three of 20 participants had one or both samples omitted from this figure: One study participant’s vomitus and stool samples are not included because only a glycerol culture for Vc was performed. One study participant’s fresh vomitus sample had no CFU on TTGA culture (and stool was TTGA Vc positive); thus, the vomitus sample was omitted from this figure. Another single study participant’s fresh stool sample had no CFU on TTGA culture (and vomitus was TTGA Vc positive); thus, the stool sample was omitted from this figure. One stool CFU count was “uncountable,” shown on this figure at 109.
See this image and copyright information in PMC

References

    1. World Health Organization. 1987. Manual for the laboratory investigations of acute enteric infections. Program for Control of Diarrheal Diseases, World Health Organization, Geneva, Switzerland.
    1. Lesmana M, Richie E, Subekti D, Simanjuntak C, Walz SE. 1997. Comparison of direct-plating and enrichment methods for isolation of Vibrio cholerae from diarrhea patients. J Clin Microbiol 35:1856–1858. doi:10.1128/jcm.35.7.1856-1858.1997. - DOI - PMC - PubMed
    1. Rennels MB, Levine MM, Daya V, Angle P, Young C. 1980. Selective vs, nonselective media and direct plating vs. enrichment technique in Isolation of Vibrio cholerae: recommendations for clinical laboratories. J Infect Dis 142:328–331. doi:10.1093/infdis/142.3.328. - DOI - PubMed
    1. Dan M, Richardson J, Miliotis MD, Koornhof HJ. 1989. Comparison of preservation media and freezing conditions for storage of specimens of faeces. J Med Microbiol 28:151–154. doi:10.1099/00222615-28-2-151. - DOI - PubMed
    1. Khan AI, Rashid MM, Islam MT, Afrad MH, Salimuzzaman M, Hegde ST, Zion MMI, Khan AH, Shirin T, Habib ZH, Khan IA, Begum YA, Azman AS, Rahman M, Clemens JD, Flora MS, Qadri F. 2020. Epidemiology of cholera in Bangladesh: findings from nationwide hospital-based surveillance, 2014–2018. Clin Infect Dis 71:1635–1642. doi:10.1093/cid/ciz1075. - DOI - PubMed

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