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. 2022 Oct 28;77(11):3205-3208.
doi: 10.1093/jac/dkac307.

A genetic cluster of OXA-244 carbapenemase-producing Escherichia coli ST38 with putative uropathogenicity factors in the Netherlands

Daan W Notermans  1 , Annelot F Schoffelen  1 , Fabian Landman  1 , Cornelia C H Wielders  1 , Sandra Witteveen  1 , Varisha A Ganesh  1 , Marga van Santen-Verheuvel  1 , Sabine C de Greeff  1 , Ed J Kuijper  1 , Antoni P A Hendrickx  1 ; Dutch CPE Surveillance Study Group
Collaborators, Affiliations

A genetic cluster of OXA-244 carbapenemase-producing Escherichia coli ST38 with putative uropathogenicity factors in the Netherlands

Daan W Notermans et al. J Antimicrob Chemother. .
No abstract available

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Figures

Figure 1.
Figure 1.
wgMLST tree of 394 carbapenemase-producing E. coli isolates showing a genetic cluster of OXA-244-producing E. coli ST38. (a) Minimum spanning tree of carbapenemase-producing E. coli from the Dutch national surveillance with EcoCluster-023 indicated with a green halo and Clermont phylotypes in multiple colours.E. coli ST38 isolates are encircled. Inset: percentage of urine-retrieved isolates per Clermont phylotype. Statistical significance is indicated, ns = not significant. (b) Genetic relationship between EcoCluster-023 isolates (marked green) is indicated by allelic differences, each circle represents an isolate and a large circle represents >1 isolate. The year of isolation is marked in the circles. Isolation from urine is indicated in yellow, while rectal swabs are indicated in red. The minimum spanning tree was based on an in-house E. coli wgMLST as described previously. The wgMLST cluster was defined as ≥2 isolates of which the genetic distance was  ≤25 alleles (25/4503,  ≤0.55% different). (c) Molecular characteristics of EcoCluster-023 isolates. Plasmid-localized antimicrobial resistance (AMR) genes are indicated in blue, chromosomal in red, uropathogenicity factors in yellow, other virulence and AMR determinants in black. Asterisks indicate genes implicated in uropathogenicity from Spurbeck et al. (d) Number of PUFs per isolate plotted per Clermont phylogroup. Kruskal–Wallis non-parametric test was performed using GraphPad Prism 9.0 (P < 0.0001) and statistical significance indicated by an * and in Table S1.
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