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Published Erratum
. 2022 Oct 4;119(40):e2215090119.
doi: 10.1073/pnas.2215090119. Epub 2022 Sep 30.

Correction for Belda-Palazón et al., ABA represses TOR and root meristem activity through nuclear exit of the SnRK1 kinase

No authors listed
Published Erratum

Correction for Belda-Palazón et al., ABA represses TOR and root meristem activity through nuclear exit of the SnRK1 kinase

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 1.
Fig. 1.
Impact of ABA and SnRK2s on SnRK1α1 subcellular localization and cell proliferation in the root apical meristem. (A) Meristems of Col-0, snrk2d, and snrk2d/1α1 7-d-old seedlings with or without ABA treatment (5 µM, 48 h). Arrowheads: regions used for violin plots. n = 23 or 24; P < 0.05, two-way ANOVA with Tukey’s honestly significant difference test. (Scale bar: 50 µm.) (B) Root apices of 4-d-old SnRK1α1-GFP#1 seedlings with or without ABA treatment (50 µM, 3 h). (Scale bar: 30 µm.) (C) Electron micrograph of meristematic epidermal cells with magnification (Lower) showing the cellular ultrastructure. C: cytoplasm; N: nucleus; No: nucleolus. (Scale bars: Upper, 5 µm; Lower, 1 µm.) (D) (Left) SnRK1α1-GFP in epidermal cells of 4-d-old root meristems. (Scale bar: 10 μm.) Dotted lines: nuclear boundary. (Right) SnRK1α1-GFP quantification and N/C ratios from two independent lines. n = 5 or 6; one-way ANOVA with Dunett’s test. (E) Nuclear SnRK1 activity in 8-d-old roots of the NLS-ACC reporter line with or without ABA treatment (50 µM, 3 h). (Left) Representative immunoblot and Ponceau-S staining as loading controls. (Right) Quantification of ACC phosphorylation [P(S79)-ACC/total ACC]. n = 3, two-tailed Student t test. (F) Impact of the presence (control, SnRK1α1-GFP#2) or absence (SnRK1α1-GFP#2; snrk2d) of SnRK2s on SnRK1α1-GFP localization assessed as in D. n = 8; two-tailed Student t test. (Scale bar: 10 μm). (G) SnRK2.2-GFP in epidermal cells of 4-d-old root meristems treated and quantified as in D. n = 5; two-tailed Student t test. (Scale bar: 10 μm). PI, propidium iodide; ns, nonsignificant; a.u., arbitrary units.
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