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. 2023 Feb;30(6):16694-16706.
doi: 10.1007/s11356-022-23360-7. Epub 2022 Oct 3.

Nano-bioremediation of textile industry wastewater using immobilized CuO-NPs myco-synthesized by a novel Cu-resistant Fusarium oxysporum OSF18

Osama M Darwesh  1 , Hao Li  2 , Ibrahim A Matter  3
Affiliations

Nano-bioremediation of textile industry wastewater using immobilized CuO-NPs myco-synthesized by a novel Cu-resistant Fusarium oxysporum OSF18

Osama M Darwesh et al. Environ Sci Pollut Res Int. 2023 Feb.

Abstract

Currently, bionanotechnologies are attracting great interest due to their promising results and potential benefits on many aspects of life. In this study, the objectives was to biosynthesis CuO-NPs using cell-free extract(s) of copper-resistant fungi and use them in bioremediation of textile industry wastewater. Out of 18 copper-resistant fungal isolates, the novel fungus strain Fusarium oxysporum OSF18 was selected for this purpose. This strain showed a high efficiency in extracellular reducing copper ions to their nano-form. The myco-synthesized CuO-NPs were characterized using UV-Vis spectroscopy, HRTEM, FTIR, and XRD and were found to be spherical nanocrystals with the size range of 21-47 nm. The bio-synthesized CuO-NPs showed promising antimicrobial activity as well as high efficiency in removing heavy metals and textile dye from industrial wastewater. The myco-synthesized CuO-NPs immobilized in alginate beads exhibited superior microbial disinfection (99.995%), heavy metals removal (93, 55, and 30 % for Pb, Cr, and Ni, respectively), and dye decolorization (90%). Such results represent a promising step to produce an eco-friendly, cost-effective, and easy-to handle tool for the bioremediation of textile industry wastewater.

Keywords: Antibacterial activity; Fusarium oxysporum OSF18; Industrial wastewater; Myco-synthesized CuO-NPs; Nano-bioremediation; Textile dyes.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Copper ions reducing and nanostructure formation by fungal isolates at 5000 ppm of copper sulfate
Fig. 2
Fig. 2
Phylogenetic tree of F. oxysporum (OSF18) with the close related reference strains
Fig. 3
Fig. 3
Production and HRTEM characterization of fungal CuO-NPs: 1, fungal culture; 2, culture filtrate; 3, CuSO4 solubilization; 4, mixture of Cu ions with filtrate; 5, proteins or reducing agents; 6, reducing agents coating ions; 7, reducing Cu ions; 8, CuO-NPs; 9, HRTEM picture
Fig. 4
Fig. 4
FTIR analyses of the myco-synthesized CuO-NPs
Fig. 5
Fig. 5
XRD analysis of the myco-synthesized CuO-NPs
Fig. 6
Fig. 6
SEM/EDAX characterization of fungal CuO-NPs
Fig. 7
Fig. 7
Antimicrobial activity of CuO-NPs (200 mg mL−1) produced by F. oxysporum (OSF18)
Fig. 8
Fig. 8
Removal efficiency of Pb, Cr, Ni, and reactive azo red dye from synthetic wastewater by CuO-NPs (10 mg mL−1) biosynthesized by F. oxysporum (OSF18)
Fig. 9
Fig. 9
Removal efficiencies of some contaminants from raw textile industry wastewater using biosynthesized CuO-NPs immobilized in alginate beads after 24 h
See this image and copyright information in PMC

References

    1. Abboud MA (2021) A novel biological approach to copper nanoparticles synthesis: characterization and its application against phytopathogenic fungi
    1. Ahmed SF, Mofijur M, Rafa N, Chowdhury AT, Chowdhury S, Nahrin M, Saiful Islam ABM, Ong HC. Green approaches in synthesising nanomaterials for environmental nanobioremediation: technological advancements, applications, benefits and challenges. Envir Res. 2022;204:111967. doi: 10.1016/j.envres.2021.111967. - DOI - PubMed
    1. Anjum M, Miandad R, Waqas M, Gehany F, Barakat M. Remediation of wastewater using various nano-materials. Arabian J Chem. 2019;12(8):4897–4919. doi: 10.1016/j.arabjc.2016.10.004. - DOI
    1. Arya A, Gupta K, Chundawat TS, Vaya D (2018) Biogenic synthesis of copper and silver nanoparticles using green alga Botryococcus braunii and its antimicrobial activity. Bioinorg Chem Appl, 2018 - PMC - PubMed
    1. Chung W, Shim J, Ravindran B. Application of wheat bran based biomaterials and nano-catalyst in textile wastewater. J King Saud Univ Sci. 2022;34(2):101775. doi: 10.1016/j.jksus.2021.101775. - DOI

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