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. 2022 Jul-Aug;25(4):440-443.
doi: 10.4103/jcd.jcd_189_22. Epub 2022 Aug 2.

Comparative evaluation of eighth-generation bonding agent modified with 7% arginine and 0.12% chitosan for antibacterial property and microtensile bond strength

Himali Rajan Desai  1 , Sanjyot A Mulay  1 , Ronit R Shinde  1 , Pradeep K Shetty  1 , Soumya S Shetty  1
Affiliations

Comparative evaluation of eighth-generation bonding agent modified with 7% arginine and 0.12% chitosan for antibacterial property and microtensile bond strength

Himali Rajan Desai et al. J Conserv Dent. 2022 Jul-Aug.

Abstract

Aim: The aim of this study was to evaluate and compare eighth-generation bonding agent (G-Premio BOND) modified with 7% arginine and 0.12% chitosan for antibacterial property and microtensile bond strength.

Methods: To evaluate antibacterial property, the sterile 96-microtiter plate was taken. The sidewalls of nine wells were coated with 15 μl of adhesive from each group. Suspension of Streptococcus mutans bacteria was placed on each group of adhesive and antibacterial property was checked using Spectrophotometer. Thirty-three healthy extracted premolars were taken and adhesive from each group was applied on the exposed surface of dentin, and the specimens were tested using a universal testing machine at crosshead speed of 1 mm/min.

Results: The least antibacterial efficacy was seen with unmodified eighth-generation bonding agent. This result was statistically significant when all study groups were compared. Microtensile bond strength was evaluated and the highest mean value (5.07) was seen with adhesive modified with 7% arginine, followed by adhesive modified with 0.12% chitosan (mean value: 4.14), and unmodified adhesive had the lowest mean bond strength value (4.07).

Conclusions: The eighth-generation bonding agent modified with 7% arginine and 0.12% chitosan showed antibacterial efficacy against S. mutans. In addition to this, they also had higher tensile bond strength values as compared to unmodified adhesive.

Keywords: Adhesive; antibacterial property; arginine; chitosan; microtensile bond strength.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Evaluation of antibacterial property: (a) Microtiter plate with study samples, (b) Wells coated with adhesive, (c) Light curing, (d and e) suspension of Streptococcus mutans bacteria placed on adhesive, (f) Testing of antibacterial property using spectrophotometer
Figure 2
Figure 2
Evaluation of microtensile bond strength: (a) Extracted premolars, (b) Etching, application of bonding agent followed by composite buildup, (c and d) Sectioning of the teeth, (e) Testing of microtensile bond strength using universal testing machine
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