Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct 5;12(1):16651.
doi: 10.1038/s41598-022-21002-z.

Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles

Teresa Faddetta  1 , Alberto Vassallo  2 , Sara Del Duca  3 , Giuseppe Gallo  1 , Renato Fani  3 , Anna Maria Puglia  1
Affiliations

Unravelling the DNA sequences carried by Streptomyces coelicolor membrane vesicles

Teresa Faddetta et al. Sci Rep. .

Abstract

Membrane vesicles (MVs) are spherical particles with nanoscale dimensions and characterized by the presence of diverse cargos, such as nucleic acids, proteins, lipids, and cellular metabolites. Many examples of (micro)organisms producing MVs are reported in literature. Among them, bacterial MVs are of particular interest because they are now considered as the fourth mechanism of horizontal gene transfer. Streptomyces bacteria are well-known for their ecological roles and ability to synthesize bioactive compounds, with Streptomyces coelicolor being the model organism. It was previously demonstrated that it can produce distinct populations of MVs characterized by different protein and metabolite cargos. In this work we demonstrated for the first time that MVs of S. coelicolor carry both DNA and RNA and that their DNA content represents the entire chromosome of the bacterium. These findings suggest that MV DNA could have a role in the evolution of Streptomyces genomes and that MVs could be exploited in new strain engineering strategies.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Electrophoretic analysis of the nucleic acids associated to fractions F3 and F4 of S. coelicolor MVs. M: DNA Molecular Weight Marker IV (Roche).
Figure 2
Figure 2
Map of S. coelicolor A3(2) M145 chromosome. Positions of katA2, catC, dnaK, hrdB, and rrnB are reported. The map was prepared with the software SnapGene Viewer using the sequence of S. coelicolor A3(2) available in GenBank with accession number AL645882.2.
Figure 3
Figure 3
Mapping of sequencing reads on the reference chromosome sequence of S. coelicolor A3(2). (A) Sequence coverage of S. coelicolor A3(2) chromosome. (B) Zoom of the region comprised between positions 5,100,000 and 5,130,000 of the reference genome AL645882.2.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Brown L, Wolf JM, Prados-Rosales R, Casadevall A. Through the wall: Extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nat. Rev. Microbiol. 2015;13:620–630. doi: 10.1038/nrmicro3480. - DOI - PMC - PubMed
    1. Hoefler BC, et al. A link between linearmycin biosynthesis and extracellular vesicle genesis connects specialized metabolism and bacterial membrane physiology. Cell Chem. Biol. 2017;24:1238–1249.e7. doi: 10.1016/j.chembiol.2017.08.008. - DOI - PubMed
    1. Kim JH, Lee J, Park J, Gho YS. Gram-negative and Gram-positive bacterial extracellular vesicles. Semin. Cell Dev. Biol. 2015;40:97–104. doi: 10.1016/j.semcdb.2015.02.006. - DOI - PubMed
    1. Faddetta T, et al. Streptomyces coelicolor vesicles: Many molecules to be delivered. Appl. Environ. Microbiol. 2022;88:e01881–e1921. doi: 10.1128/AEM.01881-21. - DOI - PMC - PubMed
    1. Gill S, Catchpole R, Forterre P. Extracellular membrane vesicles in the three domains of life and beyond. FEMS Microbiol. Rev. 2019;43:273–303. doi: 10.1093/femsre/fuy042. - DOI - PMC - PubMed

Publication types