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. 2022 Dec 31;17(1):2120303.
doi: 10.1080/15592324.2022.2120303.

Characterization of PsmiR319 during flower development in early- and late-flowering tree peonies cultivars

Chenjie Zhang  1 , Jiajia Shen  1 , Can Wang  1 , Zhanying Wang  2 , Lili Guo  1 , Xiaogai Hou  1
Affiliations

Characterization of PsmiR319 during flower development in early- and late-flowering tree peonies cultivars

Chenjie Zhang et al. Plant Signal Behav. .

Abstract

The flowering period is the most important ornamental trait of tree peony, while industrial development of tree peony has been limited by short flowering period. miR319 plays an important regulatory role in plant flowering. In the current study, the expression characteristics and evolution of PsmiR319 in tree peony flowering was explored using 'Feng Dan' and 'Lian He', which are early-flowering and late-flowering varieties of tree peony, respectively. The structure, evolution, and target(s) of PsmiR319 were analyzed by bioinformatics. Evolution analysis showed that pre-PsmiR319 was distributed in 41 plant species, among which the length of the precursor sequence exhibited marked differences (between 52 and 308 bp). Pre-PsmiR319 of tree peony was located close to the corresponding sequences of Linum usitatissimum and Picea abies in the phylogenetic tree, and in addition, could form a typical hairpin structure including a mature body with a length of 20 bp located on the 3p arm and part of the loop sequence. The mature sequence of miR319 was highly conserved among different species. Target genes of PsmiR319 include MYB-related transcription factor in tree peony. Expression of PsmiR319, assayed by qRT-PCR, differed between 'Feng Dan' and 'Lian He' during different flower development periods. PsmiR319 and its target gene showed a negative expression regulation relationship during the periods of CE (color exposure), BS (blooming stage), IF (initial flowering), and HO (half opening) in the early-flowering 'Feng Dan', and the same in FB (Full blooming) periods of late-flowering 'Lian He'. Findings from this study provide a reference for further investigation into the mechanism of miR319 in the development of different varieties of tree peony.

Keywords: PsmiR319; Tree peony; early and late flowering; expression analysis; flower development.

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Conflict of interest statement

We declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1.
Figure 1.
Form characteristics during different flower development stages of two varieties of tree peony.
Figure 2.
Figure 2.
Phylogenetic tree of pre-miR319 sequences from different plant species.
Figure 3.
Figure 3.
Multi-sequence comparison results of mature miR319 in plants.
Figure 4.
Figure 4.
Mature and precursor sequence and the predicted stem-loop structures of PsmiR319 in tree peony.
Figure 5.
Figure 5.
Target-plots of PsmiR319-regulated mRNA and degradome sequencing.
Figure 6.
Figure 6.
Specific nucleotide sequence of psu.T.00032704 that cleaved by PsmiR319.
Figure 7.
Figure 7.
Relative expression of PsmiR319 and its target gene among different flower development stages of tree peony.
See this image and copyright information in PMC

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