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Multicenter Study
. 2022 Oct 6;12(1):16790.
doi: 10.1038/s41598-022-21279-0.

Vitamin D status and determinants in Indian children and adolescents: a multicentre study

Anuradha Khadilkar  1   2 , Neha Kajale  3   4 , Chirantap Oza  3 , Rashmi Oke  3 , Ketan Gondhalekar  3 , Vivek Patwardhan  3 , Vaman Khadilkar  3   4 , Zulf Mughal  5 , Raja Padidela  5
Affiliations
Multicenter Study

Vitamin D status and determinants in Indian children and adolescents: a multicentre study

Anuradha Khadilkar et al. Sci Rep. .

Abstract

Studies performed on Indian children to assess vitamin-D status have been on small sample sizes, limited to specific geographical locations and used non-standard methods to measure 25(OH)D3. This multicentre study assessed 25(OH)D3 concentrations from dried blood spots (DBS) in 5-18-year-old Indian children and adolescents using a standardized protocol and identified factors contributing towards vitamin D deficiency. Cross-sectional, observational school-based study was conducted by multi-stage stratified random sampling. A city and nearby village were selected from 6 Indian states covering wide geographical areas. Demography, anthropometry, body-composition, dietary-intakes and DBS samples were collected. 25(OH)D3 was assessed from DBS using Liquid chromatography with tandem-mass spectrometry. Vitamin-D status was assessed in 2500 children; with additional data collected on a subset (n = 669) to assess predictors. Mean vitamin-D concentration was 45.8 ± 23.9 nmol/L, 36.8% of subjects had sufficient vitamin-D (> 50 nmol/L); rural subjects and boys had higher concentrations (p < 0.05). On regression analysis, younger age, female-gender, overweight and urban residence significantly contributed to deficiency. More than half the Indian children/adolescents were vitamin-D deficient or insufficient. Our study reinforces vitamin-D deficiency as a major public health problem and the need for supplementation, food fortification and educating the population as initiatives required to improve sufficiency status.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Flow diagram of study participants.
Figure 2
Figure 2
State wise comparison of mean (± SE) Vitamin D concentrations stratified by urban rural residence and gender. p values are displayed in Table 1 between urban and rural counterparts.
Figure 3
Figure 3
Vitamin D status (proportion deficient, insufficient, sufficient) in urban rural boys and girls from 6 Indian States.
Figure 4
Figure 4
(a–c) Indian state wise prevalence of vitamin D-deficiency, insufficiency and sufficiency. (Created with mapchart.net (https://www.mapchart.net/india.html)).
See this image and copyright information in PMC

References

    1. Bouillon R, et al. Skeletal and extra skeletal actions of vitamin D: Current evidence and outstanding questions. Endocr. Rev. 2019;40(4):1109–1151. doi: 10.1210/er.2018-00126. - DOI - PMC - PubMed
    1. Misra M, Pacaud D, Petryk A, Collett-Solberg PF, Kappy M, Drug and Therapeutics Committee of the Lawson Wilkins Pediatric Endocrine Society Vitamin D deficiency in children and its management: Review of current knowledge and recommendations. Pediatrics. 2008;122(2):398–417. doi: 10.1542/peds.2007-1894. - DOI - PubMed
    1. From Indian Academy of Pediatrics ‘Guideline for Vitamin D and Calcium in Children’ Committee. Khadilkar A, et al. Prevention and treatment of vitamin D and calcium deficiency in children and adolescents: Indian Academy of Pediatrics (IAP) Guidelines. Indian Pediatr. 2017;54(7):567–573. doi: 10.1007/s13312-017-1070-x. - DOI - PubMed
    1. Gupta P, et al. Indian Academy of Pediatrics Revised (2021) guidelines on prevention and treatment of vitamin D deficiency and rickets. Indian Pediatr. 2022;59(2):142–158. doi: 10.1007/s13312-022-2448-y. - DOI - PubMed
    1. Palacios C, Gonzalez L. Is vitamin D deficiency a major global public health problem? J. Steroid. Biochem. Mol. Biol. 2014;144(Pt A):138–145. doi: 10.1016/j.jsbmb.2013.11.003. - DOI - PMC - PubMed

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