Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Nov;106(21):6977-6992.
doi: 10.1007/s00253-022-12215-3. Epub 2022 Oct 7.

Halomonas spp., as chassis for low-cost production of chemicals

Guo-Qiang Chen  1   2   3   4 , Xu Zhang  5   6 , Xu Liu  7 , Weiran Huang  8 , Zhengwei Xie  8 , Jing Han  9 , Tong Xu  9 , Ruchira Mitra  9   10 , Cheng Zhou  9 , Jing Zhang  11 , Tao Chen  11
Affiliations
Review

Halomonas spp., as chassis for low-cost production of chemicals

Guo-Qiang Chen et al. Appl Microbiol Biotechnol. 2022 Nov.

Abstract

Halomonas spp. are the well-studied platform organisms or chassis for next-generation industrial biotechnology (NGIB) due to their contamination-resistant nature combined with their fast growth property. Several Halomonas spp. have been studied regarding their genomic information and molecular engineering approaches. Halomonas spp., especially Halomonas bluephagenesis, have been engineered to produce various biopolyesters such as polyhydroxyalkanoates (PHA), proteins including surfactants and enzymes, small molecular compounds including amino acids and derivates, as well as organic acids. This paper reviews all the progress reported in the last 10 years regarding this robust microbial cell factory. KEY POINTS: • Halomonas spp. are robust chassis for low-cost production of chemicals • Genomic information of some Halomonas spp. has been revealed • Molecular tools and approaches for Halomonas spp. have been developed • Halomonas spp. are becoming more and more important for biotechnology.

Keywords: Ectoine; Halomonas; Halophile; Metabolic engineering; Morphology engineering; PHA; PHB; Polyhydroxyalkanoates; Synthetic biology.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Afendra AS, Vargas C, Nieto JJ, Drainas C (2004) Gene transfer and expression of recombinant proteins in moderately halophilic bacteria. In: Balbás P, Lorence A (eds) Recombinant gene expression, Methods in Molecular Biology, vol 267, Humana Press, pp 209–223
    1. Argandoña MVC, Reina-Bueno M, Rodríguez-Moya J, Salvador M and Nieto J (2012) An extended suite of genetic tools for use in bacteria of the Halomonadaceae: an overview. Methods Mol Biol 824:167–201 - PubMed
    1. Becker J, Zelder O, Hafner S, Schroder H, Wittmann C (2011) From zero to hero–design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. Metab Eng 13:159–168
    1. Cai L, Tan D, Aibaidula G, Dong XR, Chen JC, Tian WD and Chen GQ (2011) Comparative genomics study of polyhydroxyalkanoates (PHA) and ectoine relevant genes from Halomonas sp. TD01 revealed extensive horizontal gene transfer events and co-evolutionary relationships. Microb Cell Fact 10: 88
    1. Camacho DM, Collins KM, Powers RK, Costello JC, Collins JJ (2018) Next-generation machine learning for biological networks. Cell 173:1581–1592 - PubMed

LinkOut - more resources