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. 2022 Jul 1;51(6):593-597.
doi: 10.1097/MPA.0000000000002077.

Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Type 1 Diabetes in Acute Pancreatitis Consortium

Clive Wasserfall  1 , Anne-Marie Dyer  2 , Cate Speake  3 , Dana K Andersen  4 , Kendall Thomas Baab  2 , Melena D Bellin, James R Broach  5 , Martha Campbell-Thompson  1 , Vernon M Chinchilli  2 , Peter J Lee  6 , Walter G Park  7 , Richard E Pratley  8 , Jami L Saloman  9 , Emily K Sims  10 , Gong Tang  11 , Dhiraj Yadav, Cemal Yazici  12 , Darwin L Conwell  6 ; Type 1 Diabetes in Acute Pancreatitis Consortium (T1DAPC)
Affiliations

Standard Operating Procedures for Biospecimen Collection, Processing, and Storage: From the Type 1 Diabetes in Acute Pancreatitis Consortium

Clive Wasserfall et al. Pancreas. .

Abstract

Differences in methods for biospecimen collection, processing, and storage can yield considerable variability and error. Therefore, best practices for standard operating procedures are critical for successful discovery, development, and validation of disease biomarkers. Here, we describe standard operating procedures developed for biospecimen collection during the DREAM (Diabetes RElated to Acute pancreatitis and its Mechanisms) Study within the Type 1 Diabetes in Acute Pancreatitis Consortium. Notably, these protocols were developed using an integrative process based on prior consortium experience and with input from working groups with expertise in immunology, pancreatitis, and diabetes. Publication and adoption consistent biospecimen protocols will inform future studies and allow for better comparisons across different metabolic research efforts.

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Conflict of interest statement

D.K.A. is part of advisory board for National Pancreas Foundation and American Pancreatic, Association Foundation and receives royalties from McGraw-Hill. M.D.B receives research support from Viacyte and Dexcom and is part of advisory board for Insulet. P.J.L. is part of advisory board for AbbVie. R.E.P. has consulting activities with Bayer AG, Corcept Therapeutics Incorporated, Dexcom, Gasherbrum Bio, Inc, Hanmi Pharmaceutical Co, Hengrui (USA) Ltd, Merck, Novo Nordisk, Pfizer, Rivus, Pharmaceuticals, Inc, Sanofi, Scohia Pharma, Inc, and Sun Pharmaceutical Industries; receives research support from Hanmi Pharmaceutical Co, Janssen, Metavention, Novo Nordisk, Poxel, SA, and Sanofi; and receives speaking fees from Novo Nordisk. E.K.S. receives speaking fees from MedScape. C.S. is part of advisory board for Vertex Pharmaceuticals. The other authors declare no conflict of interest.

Figures

FIGURE 1.
FIGURE 1.
The overall flow of biospecimens and data within the T1DAPC. DKCR, NIDDK Central Repository; PBMC, peripheral blood mononuclear cells; PSU-IPM, Penn State University Institute for Personalized Medicine; TBD, to be determined.
FIGURE 2.
FIGURE 2.
Blood sample collection.
See this image and copyright information in PMC

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