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. 2022 Oct 7;54(6):336.
doi: 10.1007/s11250-022-03332-2.

First detection of emerging HoBi-like Pestivirus (BVD-3) among some persistently infected dairy cattle herds in Egypt

Ahmed F Afify  1 , Rabab T Hassanien  2 , Hala K Abdelmegeed  2 , Ebtsam A Abouelyazeed  2 , M H Ali  2 , Dina A Abdelwahed  2 , Tahani S Behour  3
Affiliations

First detection of emerging HoBi-like Pestivirus (BVD-3) among some persistently infected dairy cattle herds in Egypt

Ahmed F Afify et al. Trop Anim Health Prod. .

Abstract

Bovine viral diarrhoea virus (BVDV) is a serious veterinary health concern worldwide. We conducted this study to determine the prevalence of persistent infections (PI) and identify the current strain among some dairy cattle herds in Egypt. A total of 240 serum samples were collected from six Egyptian provinces. Between 2019 and 2020, samples were tested by Enzyme linked immunosorbent assay (ELISA) for detection of PI animals, and then molecular characterization was performed. Six calves were found PI with a prevalence of 2.5% (6/240). Using molecular characterization, HoBi-like Pestivirus (BVD-3) was successfully identified in Egypt for the first time. Based on the BVD-3 reference strains on Genbank, the detected strains had an identity ranging from 98.8 to 99.6%. Partial nucleotide sequence of the 5'UTR gene for six tested samples was submitted to Genbank with accessions: OM324396, OM324397, OM324398, OM324399, OM3243100, and OM3243101.

Keywords: BVD; ELISA; HoBi-like Pestivirus; Persistent infection; RT-PCR.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The phylogenetic tree was created by MEGA 6 software using the maximum likelihood method. The confidence level of the neighbour-joining tree was evaluated by bootstrapping using 1000 replicates. The red dots refer to the samples in our study, with their names and accession numbers
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