Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Jun 25;51(3):350-361.
doi: 10.3724/zdxbyxb-2022-0046.

Influencing factors of pulp-dentin complex regeneration and related biological strategies

Jielin Zou  1   2   3 , Jing Mao  1   2   3 , Xin Shi  1   2   3
Affiliations
Review

Influencing factors of pulp-dentin complex regeneration and related biological strategies

Jielin Zou et al. Zhejiang Da Xue Xue Bao Yi Xue Ban. .

Abstract

Regenerative endodontic therapy (RET) utilizing tissue engineering approach can promote the regeneration of pulp-dentin complex to restore pulp vascularization, neuralization, immune function and tubular dentin, therefore the regenerated pulp-dentin complex will have normal function. Multiple factors may significantly affect the efficacy of RET, including stem cells, biosignaling molecules and biomaterial scaffolds. Stem cells derived from dental tissues (such as dental pulp stem cells) exhibit certain advantages in RET. Combined application of multiple signaling molecules and activation of signal transduction pathways such as Wnt/β-catenin and BMP/Smad play pivotal roles in enhancing the potential of stem cell migration, proliferation, odontoblastic differentiation, and nerve and blood vessel regeneration. Biomaterials suitable for RET include naturally-derived materials and artificially synthetic materials. Artificially synthetic materials should imitate natural tissues for biomimetic modification in order to realize the temporal and spatial regulation of pulp-dentin complex regeneration. The realization of pulp-dentin complex regeneration depends on two strategies: stem cell transplantation and stem cell homing. Stem cell homing strategy does not require the isolation and culture of stem cells in vitro, so is better for clinical application. However, in order to achieve the true regeneration of pulp-dentin complex, problems related to improving the success rate of stem cell homing and promoting their proliferation and differentiation need to be solved. This article reviews the influencing factors of pulp-dentin complex regeneration and related biological strategies, and discusses the future research direction of RET, to provide reference for clinical translation and application of RET.

Keywords: Biomaterial scaffold; Biosignaling molecule; Cell homing; Cell transplantation; Pulp-dentin complex regeneration; Review; Stem cells.

PubMed Disclaimer

Conflict of interest statement

所有作者均声明不存在利益冲突

Similar articles

See all similar articles

Cited by

References

    1. 刘斌, 梁景平. 牙髓再生的临床应用与未来[J]. 中华口腔医学杂志, 2020, 55 (1): 50-55 - PubMed
    2. LIU Bin, LIANG Jingping. Regenerative endodontics:clinical application status and future perspective[J]. Chinese Journal of Stomatology, 2020, 55(1): 50-55. (in Chinese)
    1. 凌均棨, 毛剑. 牙髓再生的研究现状与发展前景[J]. 中华口腔医学杂志, 2018, 53(6): 361-366 - PubMed
    2. LING Junqi, MAO Jian. State of the art and perspective of pulp regeneration[J]. Chinese Journal of Stomatology, 2018, 53(6): 361-366. (in Chinese) - PubMed
    1. OSTBY B N. The role of the blood clot in endodontic therapy. An experimental histologic study[J]. Acta Odontol Scand, 1961, 19: 324-353 - PubMed
    1. IWAYA S I, IKAWA M, KUBOTA M. Revascularization of an immature permanent tooth with apical periodontitis and sinus tract[J] Dent Traumatol. . 2001;17(4):185–187. doi: 10.1034/j.1600-9657.2001.017004185.x. - DOI - PubMed
    1. BANCHS F, TROPE M. Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol?[J] J Endods. . 2004;30(4):196–200. doi: 10.1097/00004770-200404000-00003. - DOI - PubMed