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. 2022 Jul 20:15:100397.
doi: 10.1016/j.fochx.2022.100397. eCollection 2022 Oct 30.

Phenolic compositions and antioxidant activities of Hippophae tibetana and H. rhamnoides ssp. sinensis berries produced in Qinghai-Tibet Plateau

Juan Wei  1 , Sha Li  1 , Tingting Su  1 , Jinmei Zhao  1 , Yumei Jiang  1 , Yury A Zubarev  2 , Yang Bi  1
Affiliations

Phenolic compositions and antioxidant activities of Hippophae tibetana and H. rhamnoides ssp. sinensis berries produced in Qinghai-Tibet Plateau

Juan Wei et al. Food Chem X. .

Abstract

Phenolic ingredients of Hippophae tibetana (Tib) and H. rhamnoides ssp. sinensis (Rha) berry from Qinghai-Tibet Plateau were identified by Ultra Performance Liquid Chromatography-triple Quadrupole Tandem Mass Spectrometry. Results demonstrated that both of them possessed high levels of total phenolic and flavonoid, and compared to Tib, Rha berry exhibited higher contents. Moreover, flavonols was the most predominant subclass in Rha berry, flavonols and flavanols were the two most abundant subclasses in Tib berry. Among them, rutin and narcissin were present in the most abundant amounts in Rha berry, while (-)-epigallocatechin was the richest substance in Tib berry. Furthermore, both phenolic extracts of sea buckthorn berry exhibited strong in vitro and cellular antioxidant properties. Rha berry extract exhibited much stronger effects because of its higher levels of phenolic and flavonoid profiles. This finding proved that the Rha berry could serve as a food source for better health with great potential antioxidant activity.

Keywords: Antioxidant activity; Flavonoids; H. rhamnoides ssp. sinensis; Hippophae tibetana; Phenolic compounds; Sea buckthorn.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Total phenolic content (TPC) and total flavonoid content (TFC) of Rha and Tib berry. Bars with no letters in common are significantly different (P < 0.05). Data are presented as mean ± SD, calculated from three replicates.
Fig. 2
Fig. 2
The total ion chromatogram of Rha (A) and Tib (B) berry phenolic extracts identified by UPLC-ESI-MS/MS. Peak: 01) gallic acid, 02) gallocatechin, 03) delphinidin 3-glucoside, 04) (−)-epigallocatechin, 05) cyanidin 3-o-rutinoside chloride, 06) procyanidin b3, 07) catechin, 08) procyanidin b2, 09) epicatechin, 10) myricetin 3-galactoside, 11) rutin, 12) quercetin 3-galactoside, 13) nicotiflorin, 14) narcissin, 15) astragalin, 16) isorhamnetin-3-o-glucoside.
Fig. 3
Fig. 3
Hierarchical Clustering of the phenolic compounds that were significantly differentially expressed in Rha and Tib berry. Each line is a phenolic component. The color ranging from blue to red means the component content increasing from low to high. Data showed in the figure are mean ± SD from three replicates. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Fig. 4
Fig. 4
Extracellular (A) and cellular (B) antioxidant activities of the phenolic extracts from Rha and Tib berries. Bars without letters represent significant differences (P < 0.05). Data showed in the figure are mean ± SD from three replicates.
Supplementary Fig. 1
Supplementary Fig. 1
Cytotoxicity of the phenolic extracts from Rha and Tib berry. Data showed in the figure are mean ± SD from three replicates.
See this image and copyright information in PMC

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