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. 2022 Sep 30:2022:6946051.
doi: 10.1155/2022/6946051. eCollection 2022.

miR-28-5p's Targeting of GAGE12I Inhibits Proliferation, Migration, and Invasion of Gastric Cancer in Vitro

Ruizhe Xu  1   2 , Qi Guo  1   2 , Peifeng Zhao  1   2 , Haiyan Lu  1   2 , Bo Zhang  1
Affiliations

miR-28-5p's Targeting of GAGE12I Inhibits Proliferation, Migration, and Invasion of Gastric Cancer in Vitro

Ruizhe Xu et al. Evid Based Complement Alternat Med. .

Retraction in

Abstract

GAGE12I is a tumor metastasis-promoting factor, which can induce gastric cancer cells to invade and migrate. We investigated the effect of miR-28-5p targeting GAGE12I on proliferation, invasion, and migration of human gastric cancer cell lines SGC-7901, AGS, and MGC-803. The expression levels of miR-28-5p and GAGE12I were detected by real-time PCR and western blot, respectively. Cell proliferation, migration, and invasion were measured by MTT and Transwell chamber. The interaction between miR-28-5p and GAGE12I was investigated by bioinformatics analysis and luciferase assay. Results showed that the expression of miR-28-5p in human gastric cancer cell lines was lower than that in normal gastric epithelial cells (P < 0.05). Overexpression of miR-28-5p suppressed cell proliferation, invasion, and migration (P < 0.05). GAGE12I was confirmed as a target of miR-28-5p. Cell proliferation, invasion, and migration were decreased in cells transfected with shGAGE12I compared with those of the scrambled group (P < 0.05). Collectively, miR-28-5p negatively regulated GAGE12I and reduced the proliferation, invasion, and migration of gastric cancer cells.

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Low expression of miR-28-5p in gastric cancer cells. Note: compared with GES-1, ∗P < 0.05.
Figure 2
Figure 2
Overexpression of miR-28-5p inhibits proliferation of gastric cancer cells. (a) Overexpression of miR-28-5p in SGC-7901 cells; compared with miR-NC, ∗P < 0.05. (b) Overexpression of miR-28-5p inhibited the proliferation of SGC-7901 cells. Compared with miR-NC, ∗P < 0.05.
Figure 3
Figure 3
Overexpression of miR-28-5p inhibits invasion and migration of gastric cancer cells. (a) Cell migration test, ∗P < 0.05 compared with miR-NC. (b) Cell invasion test; compared with miR-NC, ∗P < 0.05.
Figure 4
Figure 4
miR-28-5p targets GAGE12I. (a) The 3′UTR of GAGE12I contains a nucleotide sequence complementary to miR-28-5p. (b) Luciferase reporter gene test; compared with miR-NC, ∗P < 0.05. (c) Western blot was used to determine the expression of GAGE12I protein in gastric cancer cells after overexpression of miR-28-5p. (d) GAGE12I protein expression. Compared with miR-NC, ∗P < 0.05.
Figure 5
Figure 5
Knockdown GAGE12I inhibits the proliferation of gastric cancer cells. (a) Western blot was used to determine the knockdown effect. (b) GAGE12I protein expression; compared with scrambled, ∗P < 0.05. (c) Knocking down GAGE12I on the survival rate of gastric cancer cells. Compared with scrambled, ∗P < 0.05.
Figure 6
Figure 6
Knockdown GAGE12I inhibits invasion and migration of gastric cancer cells. (a) Knocking down GAGE12I on gastric cancer cell migration; compared with scrambled, ∗P < 0.05. (b) Knockdown of GAGE12I on gastric cancer cell invasion. Compared with scrambled, ∗P < 0.05.
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