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. 2022 Oct 7;45(3):e20220117.
doi: 10.1590/1678-4685-GMB-2022-0117. eCollection 2022.

Development and identification of an elite wheat-Hordeum californicum T6HcS/6BL translocation line ND646 containing several desirable traits

Zhangjun Wang  1   2 , Qingfeng Li  2 , Caixia Liu  2 , Fenglou Liu  2 , Nali Xu  2 , Mingming Yao  2 , Huixia Yu  2 , Yanqing Wang  2 , Jiajing Chen  2 , Shuangyu Bai  2 , Jingxin Yang  2 , Gang Sun  2 , Jiaohui Long  2 , Yalei Fan  2 , Ling Kang  3 , Hongxia Li  3 , Xiaogang Zhang  2 , Shengxiang Liu  2
Affiliations

Development and identification of an elite wheat-Hordeum californicum T6HcS/6BL translocation line ND646 containing several desirable traits

Zhangjun Wang et al. Genet Mol Biol. .

Abstract

Hordeum californicum (H. californicum, 2n=2X=14, HcHc), one of the wild relatives of wheat (Triticum aestivum L.), harbors many desirable genes and is a potential genetic resource for wheat improvement. In this study, an elite line ND646 was selected from a BC4F5 population, which was developed using 60Co-γ irradiated wheat-H. californicum disomic addition line WJ28-1 (DA6Hc) as the donor parent and Ningchun 4 as the recurrent parent. ND646 was identified as a novel wheat-H. californicum 6HcS/6BL translocation line using genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and H. californicum-specific expressed sequence tag (EST) markers. Further evaluation revealed that ND646 had excellent performance in several traits, such as a higher sedimentation value (SV), higher water absorption rate (WAR), and higher hardness index (HI). More importantly, it had more kernels per spike (KPS), a higher grain yields (GY), and good resistance to powdery mildew, leaf rust, and 2,4-D butylate (2,4-D). Its excellent phenotypic performance laid the foundation for further investigation of its genetic architecture and makes ND646 a useful germplasm resource for wheat breeding.

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Conflict of interest statement

Conflict of Interest: The authors declare no conflict of interest.

Figures

Figure 1 -
Figure 1 -. Scheme of the development of whea-tH.californicum translocation line ND646.
Figure 2 -
Figure 2 -. Sequential GISH/FISH analysis of the wheat- H.californicum translocation line ND646. a. GISH of ND646, the genome DNA of H.californicum was visualized in bright green; b. FISH of ND646 using pSc119.2 (shown in red) and pAs1 (shown in green) repetitive DNA as probes, white arrows show the translocation chromosome; c. GISH and FISH diagram of translocation chromosomes in DN646, respectively; d. There were 21 bivalents containing one translocation bivalent (shown in green) at the metaphase I (MI) stage in pollen mother cells (PMCs) of ND646 (Scale bar = 20µm).
Figure 3 -
Figure 3 -. Amplification of EST-PCR primer pairs in the parents and ND646. a-d. Markers CINAU 91, CINAU 502, CINAU 506 and CINAU 511, respectively. 1-4 were CS, WJ28-1, ND646 and Ningchun 4, respectively; 5-20 were the amplification of marker CINAU 91 in 16 individual plants of ND646; M was a DNA marker with a molecular weight of 100, 250, 500, 750, 1000 and 2000 bp. Arrows showed specific banding of 6HcS and 6HcL chromosomes.
Figure 4 -
Figure 4 -. Responses to diseases and herbicides of Ningchun 4 and ND646. 1 and 2 represent Ningchun 4 and ND646, respectively. a. Powdery mildew on flag leaves; b. Leaf rust on flag leaves; c. Effects of 2,4-D on spikes and whole plants, respectively.
Figure 5 -
Figure 5 -. Dynamics of grain volume (GV), fresh grain weight (FGW), dry grain weight (DGW), and grain moisture (GM) of Ningchun 4 and ND646 during grain filling.
Figure 6 -
Figure 6 -. Dynamics of grain filling rate of Ningchun 4 and ND646.
See this image and copyright information in PMC

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