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. 2022 Dec;24(6):1084-1093.
doi: 10.1007/s10126-022-10169-1. Epub 2022 Oct 13.

Silencing Asian Seabass gab3 Inhibits Nervous Necrosis Virus Replication

Zituo Yang #  1   2 , Yepin Yu #  3 , Le Wang  1 , Sek-Man Wong  1   2   4 , Gen Hua Yue  5   6
Affiliations

Silencing Asian Seabass gab3 Inhibits Nervous Necrosis Virus Replication

Zituo Yang et al. Mar Biotechnol (NY). 2022 Dec.

Abstract

The nervous necrosis virus (NNV) causes the viral nervous necrosis (VNN) disease in aquatic animals and has been a major threat in aquaculture. Thus, it is essential for the development of a prevention method to minimize economic losses caused by NNV such as the identification of NNV resistance genes and application of these genes in molecular breeding to increase disease resistance. gab3 is an important NNV resistance gene in Asian seabass. However, the mechanism of gab3 in NNV resistance has not been elucidated. In this study, knockdown of gab3 in NNV-infected Asian seabass cells resulted in a significant decrease in viral RNA and virus titers. Knockout of gab3 in zebrafish led to an increased survival rate and resistant time after NNV infection. Cellular localization of the GAB3 and NNV by immunofluorescence staining showed that the GAB3 was translocated from the nucleus to the cytoplasm, and finally reached the cell membrane of SB cells after 48 h post NNV infection. Our study suggests that gab3 plays an important role in NNV replication and silencing gab3 can inhibit virus replication.

Keywords: CRISPR-Cas9; GAB3; Knockdown; NNV; Resistance.

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References

    1. Chang N, Sun C, Gao L, Zhu D, Xu X, Zhu X, Xiong J-W, Xi JJ (2013) Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos. Cell Res 23:465–472 - DOI - PubMed - PMC
    1. Cheng C-A, Luo J-M, Chiang M-H, Fang K-Y, Li C-H, Chen C-W, Wang Y-S, Chang C-Y (2021) Nervous necrosis virus coat protein mediates host translation shutoff through nuclear translocalization and degradation of polyadenylate binding protein. J Virol 95:e02364–e2420 - DOI - PMC
    1. Chi S, Shieh J, Lin S (2003) Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Dis Aquat Org 55:221–228 - DOI
    1. Cook KC, Cristea IM (2019) Location is everything: protein translocations as a viral infection strategy. Curr Opin Chem Biol 48:34–43 - DOI - PubMed
    1. Deng H, Fung G, Qiu Y, Wang C, Zhang J, Jin Z-G, Luo H (2017) Cleavage of Grb2-associated binding protein 2 by viral proteinase 2A during Coxsackievirus infection. Front Cell Infect Microb 7:85 - DOI

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