Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Nov;106(22):7387-7396.
doi: 10.1007/s00253-022-12229-x. Epub 2022 Oct 14.

Effects and mechanisms of animal-free hydrolysates on recombination protein yields in CHO cells

Qiujie Du  1   2 , Xi Zhang  3 , Tianyun Wang  1   2 , Xiaoyin Wang  4   5
Affiliations
Review

Effects and mechanisms of animal-free hydrolysates on recombination protein yields in CHO cells

Qiujie Du et al. Appl Microbiol Biotechnol. 2022 Nov.

Abstract

Chinese hamster ovary (CHO) cells are the commonly used cell lines for producing recombinant therapeutic proteins (RTPs) because they possess post-translational modifications similar to human cells. Culture media are necessary for cell growth, and their quality affects the yields and quality of RTPs. Due to safety concerns for the complex purification of RTPs, the development of serum-free media (SFM) is necessary for CHO cells. To meet the need for CHO cells with higher cell density and RTP productivity with consistent product quality in large-scale suspension cultures, the optimization of SFM through adding some enzymatic animal-free hydrolysates (AFHs) is preferred. The AFHs can improve cell culture performance and product yield of RTPs without affecting their quality. Here, the effect and mechanism of various AFHs in improving CHO cell culture performance and protein expression are reviewed. KEY POINTS: • AFHs that improve the recombinant protein yield of CHO cells are reviewed. • AFHs improve recombinant protein yield via influencing cell performance. • The AFHs do not affect the quality of recombinant protein in CHO cells. • AFHs can provide nutrients, block cell cycle, and reduce oxidative stress.

Keywords: Animal origin–free; CHO cells; Hydrolysate; Recombinant therapeutic proteins; Serum-free media.

PubMed Disclaimer

References

    1. Aguilar-Toalá JE, Deering AJ, Liceaga AM (2020) New insights into the antimicrobial properties of hydrolysates and peptide fractions derived from chia seed (Salvia hispanica L.). Probiotics Antimicrob Proteins 12(4):1571–1581. https://doi.org/10.1007/s12602-020-09653- - DOI - PubMed
    1. Amagliani L, O’Regan J, Kelly A, O’Mahony JA (2017) The composition, extraction, functionality and applications of rice proteins: a review. Trends Food Sci Tech 64:1–12. https://doi.org/10.1016/j.tifs.2017.01.008 - DOI
    1. Azarian B, Sajedin SM, Azimi A, Raigani M, Vaziri B, Davami F (2017) Proteomics profiling of chimeric-truncated tissue plasminogen activator producing-chinese hamster ovary cells cultivated in a chemically defined medium supplemented with protein hydrolysates. Iran Biomed J 21(3):154–166. https://doi.org/10.18869/acadpub.ibj.21.3.154
    1. Baik JY, Lee MS, An SR, Yoon SK, Joo EJ, Kim YH, Park HW, Lee GM (2006) Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. Biotechnol Bioeng 93(2):361–371. https://doi.org/10.1002/bit.20717 - DOI - PubMed
    1. Baik JY, Joo EJ, Kim YH, Lee GM (2008) Limitations to the comparative proteomic analysis of thrombopoietin producing Chinese hamster ovary cells treated with sodium butyrate. J Biotechnol 133(4):461–468. https://doi.org/10.1016/j.jbiotec.2007.11.008 - DOI - PubMed

Substances

LinkOut - more resources