Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Meta-Analysis
. 2022 Oct 13;22(1):785.
doi: 10.1186/s12879-022-07766-9.

Diagnostic accuracy of multiplex respiratory pathogen panels for influenza or respiratory syncytial virus infections: systematic review and meta-analysis

Sophie Jullien #  1 , Felicity Fitzgerald #  2 , Suzanne Keddie #  3 , Oliver Baerenbold  2 , Quique Bassat  4   5   6   7   8 , John Bradley  3 , Jane Falconer  3 , Colin Fink  9   10 , Ruth Keogh  3 , Heidi Hopkins #  3 , Marie Voice #  9
Affiliations
Meta-Analysis

Diagnostic accuracy of multiplex respiratory pathogen panels for influenza or respiratory syncytial virus infections: systematic review and meta-analysis

Sophie Jullien et al. BMC Infect Dis. .

Abstract

Respiratory syncytial virus (RSV) and influenza viruses are important global causes of morbidity and mortality. We evaluated the diagnostic accuracy of the Luminex NxTAG respiratory pathogen panels (RPPs)™ (index) against other RPPs (comparator) for detection of RSV and influenza viruses. Studies comparing human clinical respiratory samples tested with the index and at least one comparator test were included. A random-effect latent class meta-analysis was performed to assess the specificity and sensitivity of the index test for RSV and influenza. Risk of bias was assessed using the QUADAS-2 tool and certainty of evidence using GRADE. Ten studies were included. For RSV, predicted sensitivity was 99% (95% credible interval [CrI] 96-100%) and specificity 100% (95% CrI 98-100%). For influenza A and B, predicted sensitivity was 97% (95% CrI 89-100) and 98% (95% CrI 88-100) respectively; specificity 100% (95% CrI 99-100) and 100% (95% CrI 99-100), respectively. Evidence was low certainty. Although index sensitivity and specificity were excellent, comparators' performance varied. Further research with clear patient recruitment strategies could ascertain performance across different populations.Protocol Registration: Prospero CRD42021272062.

Keywords: Diagnosis; Influenza virus; Molecular diagnostics; Respiratory infection; Respiratory syncytial virus.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Risk of bias and applicability concerns in the accuracy of Luminex NxTAGG RPP™ for the detection of RSV and influenza viruses in respiratory samples
Fig. 2
Fig. 2
Sensitivity and specificity estimations for the Luminex NxTAG RPP™ versus comparator diagnostic test panels for detecting RSV in respiratory samples with their 95% credible intervals (CrI). The forest plot includes two entries for the Esposito 2016 study, one for each comparator included in that study [33]
Fig. 3
Fig. 3
Pooled (shaded) and predicted (dashed) credible regions of Luminex NxTAG RPP™ for detecting RSV in respiratory samples
Fig. 4
Fig. 4
Forest plot of the sensitivity and specificity of Luminex NxTAG RPP™ versus comparator RPP for detecting influenza virus in respiratory samples, with their 95% credible intervals (CrI)
Fig. 5
Fig. 5
Pooled (shaded) and predicted (dashed) credible regions of Luminex NxTAG RPP™ for detecting influenza viruses in respiratory samples
See this image and copyright information in PMC

References

    1. Shi T, McAllister D, O’Brien K, Simoes E, Madhi S, Gessner B, et al. Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study. Lancet. 2017;390(10098):946–958. doi: 10.1016/S0140-6736(17)30938-8. - DOI - PMC - PubMed
    1. Srikantiah P, Vora P, Klugman KP. Assessing the full burden of respiratory syncytial virus in young infants in low- and middle-income countries: The importance of community mortality studies. Clin Infect Dis. 2021;73(S3):177–179. doi: 10.1093/cid/ciab486. - DOI - PMC - PubMed
    1. Mazur N, Löwensteyn Y, Willemsen J, Gill C, Forman L, Mwananyanda L, et al. Global respiratory syncytial virus-related infant community deaths. Clin Infect Dis. 2021;73(Suppl 3):S229–S237. doi: 10.1093/cid/ciab528. - DOI - PMC - PubMed
    1. Iuliano A, Roguski K, Chang H, Muscatello D, Palekar R, Tempia S, et al. Estimates of global seasonal influenza-associated respiratory mortality: a modelling study. Lancet. 2018;391(10127):1285–1300. doi: 10.1016/S0140-6736(17)33293-2. - DOI - PMC - PubMed
    1. Wang X, Li Y, O’Brien K, Madhi S, Widdowson M, Byass P, et al. Global burden of respiratory infections associated with seasonal influenza in children under 5 years in 2018: a systematic review and modelling study. Lancet Glob Heal. 2020;8(4):e497–510. doi: 10.1016/S2214-109X(19)30545-5. - DOI - PMC - PubMed

MeSH terms