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. 2022 Sep 27;27(19):6375.
doi: 10.3390/molecules27196375.

Enhancing Resistant Starch Content of High Amylose Rice Starch through Heat-Moisture Treatment for Industrial Application

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Enhancing Resistant Starch Content of High Amylose Rice Starch through Heat-Moisture Treatment for Industrial Application

Chang-Seon Lee et al. Molecules. .

Abstract

The objective of the present study is to enhance the resistant starch (RS) content of high amylose rice starch with heat-moisture treatment (HMT) for industrial application. The optimized HMT condition for achieving the highest RS content established using response surface methodology (RSM) was a temperature of 100 °C, moisture content of 24.2%, and a time of 11.5 h. Upon HMT, the RS content increased from 32.1% for native starch to 46.4% in HMT starch with optimized condition. HMT of the starches reduced the solubility and swelling power. The surface of HMT starch granules was more irregular than native starch. The X-ray diffraction (XRD) peak intensity at 2θ = 5° was greatly reduced by HMT, and the peaks at 22.7° and 24.2° were merged. HMT increased the gelatinization temperature and reduced the gelatinization enthalpy. HMT provides a method for the production of high-yield RS2 with high amylose rice starch in industrial application.

Keywords: granular morphology; heat–moisture treatment; high amylose rice starch; physicochemical properties; resistant starch; response surface methodology.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Contour plots for the effects of two variables under a constant one variable. (AC): Treatment time was fixed for 0, 8 and 16 h, respectively. (DF): Moisture level was fixed at 15, 21 and 27%, respectively. (GI): Temperature was fixed at 100, 110 and 120 °C, respectively.
Figure 2
Figure 2
Scanning electron microphotographs of native and heat–moisture treated rice starches. NR: native rice starch, HR: heat–moisture treated rice starch, 100, 110 and 120: heat–moisture treatment temperature, 15, 21 and 27: moisture content, 8 and 16: heat–moisture treatment time.
Figure 2
Figure 2
Scanning electron microphotographs of native and heat–moisture treated rice starches. NR: native rice starch, HR: heat–moisture treated rice starch, 100, 110 and 120: heat–moisture treatment temperature, 15, 21 and 27: moisture content, 8 and 16: heat–moisture treatment time.
Figure 3
Figure 3
XRD patterns of native and heat–moisture treated rice starches. NR: native rice starch, HR: heat–moisture treated rice starch, 100, 110 and 120: heat–moisture treatment temperature, 15, 21 and 27: moisture content, 8 and 16: heat–moisture treatment time.

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