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. 2022 Oct 5;27(19):6621.
doi: 10.3390/molecules27196621.

Comparative Proteomics of Potato Cultivars with a Variable Dormancy Period

Daniel Mouzo  1 , Raquel Rodríguez-Vázquez  1 , Carlos Barrio  2 , Lucio García  2 , Carlos Zapata  1
Affiliations

Comparative Proteomics of Potato Cultivars with a Variable Dormancy Period

Daniel Mouzo et al. Molecules. .

Abstract

The control of the duration of the dormancy phase is a significant challenge in the potato industry and for seed producers. However, the proteome landscape involved in the regulation of the length of the dormancy period over potato cultivars remains largely unexplored. In this study, we performed for the first time a comparative proteome profiling of potato cultivars with differential duration of tuber dormancy. More specifically, the proteome profiling of Agata, Kennebec and Agria commercial potato varieties with short, medium and medium-long dormancy, respectively, was assessed at the endodormancy stage using high-resolution two-dimensional electrophoresis (2-DE) coupled to reversed-phase liquid chromatography-tandem mass spectrometry (LC-TripleTOF MS/MS). A total of 11 proteins/isoforms with statistically significant differential abundance among cultivars were detected on 2-DE gels and confidently identified by LC-TripleTOF MS/MS. Identified proteins have known functions related to tuber development, sprouting and the oxylipins biosynthesis pathway. Fructokinase, a mitochondrial ADP/ATP carrier, catalase isozyme 2 and heat shock 70 kDa were the proteins with the strongest response to dormancy variations. To the best of our knowledge, this study reports the first candidate proteins underlying variable dormancy length in potato cultivars.

Keywords: Solanum tuberosum; catalase; dormancy; endodormancy; hydrogen peroxide; lipid mobilization; oxylipins; paradormancy; α-ketol.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic timeline from harvest to tuber sprouting under favorable conditions. Endodormancy is the first stage of tuber dormancy where the growth of meristems is inhibited. Once endodormancy weakens, there is a transition to paradormancy, and the apical bud will grow while the other meristems will be inhibited in paradormancy. Subsequently, the release of the paradormancy, allows for the appearance of secondary shoots that together with the apical bud form the plant through normal sprouting. Furthermore, endodormancy will become ecodormancy, and growth will be inhibited under unfavorable environmental conditions (e.g., low temperatures).
Figure 2
Figure 2
Two-dimensional electrophoresis gel images of global endodormancy proteomes from three potato cultivars (Agata, Kennebec and Agria) at the endodormancy stage. Squares indicate the fourteen spots that house a single differentially abundant protein between cultivars according to LC-TripleTOF MS/MS analysis.
Figure 3
Figure 3
Principal component analysis (PCA) using the volume of 78 differentially abundant (p < 0.05) spots between Agata, Kennebec and Agria (replicates 1–4) potato cultivars at the endodormancy stage.
Figure 4
Figure 4
Simplified scheme of differentially abundant proteins between potato tubers from cultivars at the endodormancy stage involved in oxylipin biosynthesis.
See this image and copyright information in PMC

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