. 2022 Oct 14;21(1):76.

doi: 10.1186/s12938-022-01042-2.

Viability of ex-vivo myography as a diagnostic tool for rectus abdominis muscle electrical activity collected at Cesarean section within a diamater cohort study

David R A Reyes^#¹, Angelica M P Barbosa^#^{1

2}, Floriano F Juliana¹, Quiroz B C V Sofia¹, Sarah M B Costa¹, Raghavendra L S Hallur^{1

3}, Eusebio M A Enriquez¹, Rafael G Oliveira¹, Patricia de Souza Rossignolli², Cristiane Rodrigues Pedroni², Fernanda C B Alves¹, Gabriela A Garcia¹, Joelcio F Abbade¹, Carolina N F Carvalho¹, Luis Sobrevia^{1

4

5

6

7}, Marilza V C Rudge^#⁸, Iracema I M P Calderon^#¹; Diamater Study Group

Collaborators, Affiliations

Collaborators

Diamater Study Group:
F P Souza, T Lehana, C F O Graeff, C G Magalhães, R A A Costa, S A M Lima, M R K Rodrigues, S L Felisbino, W F Barbosa, F J Campos, G Bossolan, J E Corrente, H R C Nunes, P S Rossignoli, Á N Atallah, Z I Jármy-Di Bella, S M M Uchôa, M A H Duarte, E A Mareco, M E Sakalem, N M Martinho, D G Bussaneli, M I G Orlandi, C Pascon, T D Dangió, F Piculo, G M Prata, R E Avramidis, A B M Magyori, G T A Nava, T C D Caldeirão, R H L Shetty, J P C Marcondes, M L S Takemoto, C B Prudencio, F A Pinheiro, C I Sartorao Filho, S B C V Quiroz, T Pascon, S K Nunes, B B Catinelli, F V D S Reis, M O Menezes, N J Santos, L Takano, A M Carr, L F Iamundo, H C M Bassin, V P Barbosa, M Jacomin, A J B Silva, I O Lourenço, J Marosticadesá, I P Caruso, L T Rasmussen, V K C Nogueira, J T Ribeiro-Paes, D C H França, H V M Bastos, M L A Heliodoro, M N Kuroda, H L Carvalho

Affiliations

¹ Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo, CEP18618-687, Brazil.
² Department of Physiotherapy and Occupational Therapy, School of Philosophy and Sciences, São Paulo State University (UNESP), Marilia, Brazil.
³ Centre for Biotechnology, Pravara Institute of Medical Sciences (Deemed to Be University), Loni-413736, Rahata Taluk, Ahmednagar District, Ahmednagar, Maharashtra, India.
⁴ Cellular and Molecular Physiology Laboratory (CMPL), Department of Obstetrics, Division of Obstetrics and Gynaecology, School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, 8330024, Santiago, Chile.
⁵ Department of Physiology, Faculty of Pharmacy, Universidad de Sevilla, 41012, Seville, Spain.
⁶ University of Queensland Centre for Clinical Research (UQCCR), Faculty of Medicine and Biomedical Sciences, University of Queensland, Herston, QLD, 4029, Australia.
⁷ Department of Pathology and Medical Biology, Division of Pathology, University of Groningen, University Medical Center Groningen (UMCG), Groningen, The Netherlands.
⁸ Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo, CEP18618-687, Brazil. marilzarudge@gmail.com.

^# Contributed equally.

PMID: 36242084
PMCID: PMC9563120
DOI: 10.1186/s12938-022-01042-2

Viability of ex-vivo myography as a diagnostic tool for rectus abdominis muscle electrical activity collected at Cesarean section within a diamater cohort study

David R A Reyes et al. Biomed Eng Online. 2022.

. 2022 Oct 14;21(1):76.

doi: 10.1186/s12938-022-01042-2.

Authors

Collaborators

Diamater Study Group:
F P Souza, T Lehana, C F O Graeff, C G Magalhães, R A A Costa, S A M Lima, M R K Rodrigues, S L Felisbino, W F Barbosa, F J Campos, G Bossolan, J E Corrente, H R C Nunes, P S Rossignoli, Á N Atallah, Z I Jármy-Di Bella, S M M Uchôa, M A H Duarte, E A Mareco, M E Sakalem, N M Martinho, D G Bussaneli, M I G Orlandi, C Pascon, T D Dangió, F Piculo, G M Prata, R E Avramidis, A B M Magyori, G T A Nava, T C D Caldeirão, R H L Shetty, J P C Marcondes, M L S Takemoto, C B Prudencio, F A Pinheiro, C I Sartorao Filho, S B C V Quiroz, T Pascon, S K Nunes, B B Catinelli, F V D S Reis, M O Menezes, N J Santos, L Takano, A M Carr, L F Iamundo, H C M Bassin, V P Barbosa, M Jacomin, A J B Silva, I O Lourenço, J Marosticadesá, I P Caruso, L T Rasmussen, V K C Nogueira, J T Ribeiro-Paes, D C H França, H V M Bastos, M L A Heliodoro, M N Kuroda, H L Carvalho

Affiliations

¹ Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo, CEP18618-687, Brazil.
² Department of Physiotherapy and Occupational Therapy, School of Philosophy and Sciences, São Paulo State University (UNESP), Marilia, Brazil.
³ Centre for Biotechnology, Pravara Institute of Medical Sciences (Deemed to Be University), Loni-413736, Rahata Taluk, Ahmednagar District, Ahmednagar, Maharashtra, India.
⁴ Cellular and Molecular Physiology Laboratory (CMPL), Department of Obstetrics, Division of Obstetrics and Gynaecology, School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, 8330024, Santiago, Chile.
⁵ Department of Physiology, Faculty of Pharmacy, Universidad de Sevilla, 41012, Seville, Spain.
⁶ University of Queensland Centre for Clinical Research (UQCCR), Faculty of Medicine and Biomedical Sciences, University of Queensland, Herston, QLD, 4029, Australia.
⁷ Department of Pathology and Medical Biology, Division of Pathology, University of Groningen, University Medical Center Groningen (UMCG), Groningen, The Netherlands.
⁸ Department of Gynecology and Obstetrics, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, São Paulo, CEP18618-687, Brazil. marilzarudge@gmail.com.

^# Contributed equally.

PMID: 36242084
PMCID: PMC9563120
DOI: 10.1186/s12938-022-01042-2

Abstract

Background: Ex-vivo myography enables the assessment of muscle electrical activity response. This study explored the viability of determining the physiological responses in muscles without tendon, as rectus abdominis muscle (RAM), through ex-vivo myography to assess its potential as a diagnostic tool.

Results: All tested RAM samples (five different samples) show patterns of electrical activity. A positive response was observed in 100% of the programmed stimulation. RAM 3 showed greater weight (0.47 g), length (1.66 cm), and width (0.77 cm) compared to RAM 1, RAM 2, RAM 4 and RAM 5 with more sustained electrical activity over time, a higher percentage of fatigue was analyzed at half the time of the electrical activity. The order of electrical activity (Mn) was RAM 3 > RAM 5 > RAM 1 > RAM 4 > RAM 2. No electrical activity was recorded in the Sham group.

Conclusions: This study shows that it is feasible to assess the physiological responses of striated muscle without tendon as RAM, obtained at C-section, under ex vivo myography. These results could be recorded, properly analyzed, and demonstrated its potential as a diagnostic tool for rectus abdominis muscle electrical activity.

Keywords: C-section; Contractility; Myography; Rectus abdominis muscle; Skeletal muscle.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

**Fig. 1**
Four steps of the experiment performed from the RAM without tendon

**Fig. 2**
Response to the programmed electrical stimulation in five RAM samples. The myograph shows the behavior of elastic ribbon (sham) under the same electrical stimulation as RAMs; A–E represents RAM 1–5 and F represents the sham group

**Fig. 3**
Fatigue andloss of the viability of five RAM. A–E Samples at half the time of the electrical activity

See this image and copyright information in PMC

References

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1. Piculo F, Marini G, Vesentini G, Morceli G, Damasceno DC, Sobrevia L, et al. Pregnancy-specific urinary incontinence in women with gestational hyperglycaemia worsens the occurrence and severity of urinary incontinence and quality of life over the first year postpartum. Eur J Obstet Gynecol Reprod Biol. 2020;252:336–343. doi: 10.1016/j.ejogrb.2020.06.036. - DOI - PubMed
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Viability of ex-vivo myography as a diagnostic tool for rectus abdominis muscle electrical activity collected at Cesarean section within a diamater cohort study

Collaborators

Affiliations

Viability of ex-vivo myography as a diagnostic tool for rectus abdominis muscle electrical activity collected at Cesarean section within a diamater cohort study

Authors

Collaborators

Affiliations

Abstract

Conflict of interest statement

Figures

References

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Medical