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. 2022 Summer;17(3):294-302.
doi: 10.30699/IJP.2022.547555.2718. Epub 2022 Aug 13.

The Antibacterial and Anti-biofilm Traits of the Novel BMAP-27-Melittin Conjugated Peptide Nanoparticle Against Streptococcus mutans: Clinical Isolates from Oral Cavity

Mohammad Pourahmadi  1 , Kimia Pourahmadi  2 , Farzan Modaresi  3 , Shekoufeh Atashpour  3   4 , Azita Azad  5 , Alireza Ranjbaran  5 , Abdolmajid Ghasemian  6
Affiliations

The Antibacterial and Anti-biofilm Traits of the Novel BMAP-27-Melittin Conjugated Peptide Nanoparticle Against Streptococcus mutans: Clinical Isolates from Oral Cavity

Mohammad Pourahmadi et al. Iran J Pathol. 2022 Summer.

Abstract

Background & objective: The spread and development of drug-resistant bacterial strains has prompted the hunt for novel antibacterial polypeptides undergoing conformational changes to confer rapid bactericidal effects. The aim of this study was to evaluate the effect of novel BMAP27-Melittin conjugated peptide- nanoparticle (NP) against Streptococcus mutans as the primary pathogen from subgingival plaques.

Methods: Sixty subgingival plaque samples were collected, and 39 S. mutans isolates were identified. The BMAP27-Melittin conjugated peptide was purchased from GenScript Company, USA. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Biofilm Inhibitory Concentration (BIC), and Biofilm Eradication Concentration (BEC) of BMAP27-Melittin-NP were calculated using the microtiter method.

Results: Thirty-nine infected subjects were reported, including 24 males and 15 females (P=0.299). MIC, MBC, BIC, and BEC of BMAP27-Melittin-NP against S. mutans were 1.8, 2.9, 2.1, and 3.8μg/mL, respectively. The mean MBC, BEC, and BIC values were significantly lower among clinical isolates than S. mutans ATCC 35688 standard strain (P=0.032, 0.001, and 0.001, respectively).

Conclusion: BMAP27-Melittin-NP demonstrated significant antibacterial and anti-biofilm effects against clinical isolates of S. mutans which can be considered a promising compound to prevent or treat dental caries and eradicate the oral infections.

Keywords: Antimicrobial Peptide; BMAP-27; Biofilms; Melittin; Nanoparticles; Streptococcus mutans.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1
The relationship between gender and the presence of the S. mutans
Fig. 2
Fig. 2
Electrophoresis of S. mutans 16SrRNA gene product (433 bp) on 1% agarose gel; wells: M: 100 bp DNA marker, PC: positive control, NC: negative control, 1-11: positive samples
Fig. 3
Fig. 3
The mean age of S. mutans-positive and negative subjects
Fig. 4.
Fig. 4.
BMAP27-Melittin-NPs TEM image (30-40 nm size): JEOL Jem 1011 Transmission Electron Microscope. Mass concentration: Freeze Dryer Christ Alpha 1-4 LSC. Zeta Potential: Malvern Zetasizer Nano ZS9. UV-Vis: Spectrophotometer Nanodrop 2000c
Fig. 5
Fig. 5
MIC, MBC, BIC and BEC value of BMAP27-Melittin conjugated peptide NPs against S. mutans
See this image and copyright information in PMC

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