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. 2018 Aug 3;15(Suppl 1):971-983.
doi: 10.21451/1984-3143-AR2018-0043. eCollection 2018 Jul-Sep.

Assisted reproductive technologies (ART) in water buffaloes

Pietro S Baruselli  1 , Julia G Soares  2 , Bernardo M Bayeux  1 , Júlio C B Silva  1 , Rodolfo D Mingoti  1 , Nelcio A T Carvalho  3
Affiliations

Assisted reproductive technologies (ART) in water buffaloes

Pietro S Baruselli et al. Anim Reprod. .

Abstract

Our expanding knowledge of ovarian function during the buffalo estrous cycle has given new approaches for the precise synchronization of follicular development and ovulation to apply consistently assisted reproductive technologies (ART). Recent synchronization protocols are designed to control both luteal and follicular function and permit fixed-time AI with high pregnancy rates during the breeding (autumn-winter) and nonbreeding (spring- summer) seasons. Additionally, allow the initiation of superstimulatory treatments at a self-appointed time and provide opportunities to do fixed-time AI in donors and fixed-time embryo transfer in recipients. However, due the scarce results of in vivo embryo recovery in superovulated buffaloes, the association of ovum pick-up (OPU) with in vitro embryo production (IVEP) represents an alternative method of exploiting the genetics of high yeld buffaloes. Nevertheless, several factors appear to be critical to OPU/IVEP efficiency, including antral follicle population, follicular diameter, environment, farm and category of donor. This review discusses a number of key points related to the manipulation of ovarian follicular growth to improve assisted reproductive technologies in buffalo.

Keywords: artificial insemination; embryo transfer; synchronization..

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Figures

Figure 1
Figure 1. Standardized diameters of ovarian follicles (dominant follicle, largest and second largest subordinate follicle) in buffalo cows with a) two wave (n = 19) and b) three wave (n = 10) estrous cycles. Adapted from Baruselli et al. (1997).
Figure 2
Figure 2. Probability of blastocyst rate (□), pregnancy rate (●) and blastocisty per OPU (∆) as a function of numbers of retrieved oocytes per OPU in Murrah buffalo (Bubalus bubalis) donors (n = 179). Probability_blastocyst_rate = EXP (-0.0375* Oocytes_retrived -1.2673) / [1+ EXP ( -0.0375 * Oocytes_retrived - 1.2673)]; P = 0.07; r2 = 0,02 Probability_pregnancy_rate = EXP (-0.0287 * Oocytes_retrived -0.5366) / [1+ EXP (-0.0287 * Oocytes_retrived - 0.5366)]; P = 0.41; r2 = 0.0025. Probability_blastocyst per OPU = EXP (+0.0891 * Oocytes_retrived -0.7164) / [1+ EXP (+0.0891 * Oocytes_retrived - 0.7164)]; P < 0.001; r2 = 0.35.
Figure 3
Figure 3. Distribution of oocytes retrieved per OPU in Murrah buffalo donor (n = 179).
Figure 4
Figure 4. Blastocyst rate (%) according to sires used (n = 8) during IVEP from buffalo (Bubalus bubalis) donors (n = 379).
Figure 5
Figure 5. Proportion of small (<6 mm), medium (6-10 mm), and large follicles (>10 mm) in buffalo donor submitted to OPU with and without FSH superstimulation prior to OPU.
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References

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