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. 2022 Sep 28:12:1025889.
doi: 10.3389/fcimb.2022.1025889. eCollection 2022.

Clinical study on sequential treatment of severe diarrhea irritable bowel syndrome with precision probiotic strains transplantation capsules, fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules

Linling Lv  1 , Guangcong Ruan  1 , Yi Ping  1 , Yi Cheng  1 , Yuting Tian  1 , Zhifeng Xiao  1 , Xuefei Zhao  1 , Dongfeng Chen  1 , Yanling Wei  1
Affiliations

Clinical study on sequential treatment of severe diarrhea irritable bowel syndrome with precision probiotic strains transplantation capsules, fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules

Linling Lv et al. Front Cell Infect Microbiol. .

Abstract

Objective: To study the effect of precision probiotic strains transplantation capsules on diarrhea irritable bowel syndrome compared with fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules.

Methods: Two patients with severe irritable bowel syndrome were treated with precision probiotic strains transplantation capsules, fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules in sequence. IBS-SSS, IBS-QoL, GSRS, stool frequency, stool character, degree of abdominal pain, GAD-7, and PHQ9 scores of patients at 0, 2, 4, 6, 8, 10, and 12 weeks of treatment were monitored and recorded, and stool samples were collected for metagenomics and metabolomics.

Results: It was found that the IBS-SSS score of patient case 1 decreased by 175 points and that of patient case 2 decreased by 100 points after treatment of precision probiotic strains transplantation capsules. There was no significant decrease after fecal microbiota transplantation capsules and live combined bacillus subtilis and enterococcus faecium capsules were used. At the same time, compared with fecal microbiota transplantation and live combined bacillus subtilis and enterococcus faecium capsules, the IBS QoL, stool frequency, stool character, degree of abdominal pain and GAD-7 score of patient case 1 improved more significantly by the precision probiotic strains transplantation capsules. And the stool frequency and stool character score of patient case 2 decreased more significantly. Intestinal microbiota also improved more significantly after the precise capsule transplantation treatment. And we found Eubacterium_ Eligens showed the same change trend in the treatment of two patients, which may play a role in the treatment.

Conclusion: precision probiotic strains transplantation capsules is more beneficial to improve the intestinal microbiota of patients than microbiota transplantation capsule and live combined bacillus subtilis and enterococcus faecium capsules, so as to better alleviate clinical symptoms. This study provides a more perfect and convenient therapeutic drugs for the treatment of IBS.

Keywords: Irritable bowel syndrome; fecal microbiota transplantation capsules; gut microbiota; live combined bacillus subtilis and enterococcus faecium capsules; precision transplantation.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Change trend of IBS clinical indicators of patient case 1. (A). IBS-SSS score. (B). stool frequency. (C). Character of stool. (D). Abdominal pain score. (E). GSRS. (F). IBS-QoL. (G). GAD7. (H). PHQ9.
Figure 2
Figure 2
Change trend of IBS clinical indicators of patient case 2. (A). IBS-SSS score. (B). stool frequency. (C). Character of stool. (D). Abdominal pain score. (E). GSRS. (F). IBS-QoL. (G). GAD7. (H). PHQ9.
Figure 3
Figure 3
Characteristics of gastrointestinal microbiota in patient case 1. (A). Composition of the top 10 species at the species level. (B). The diversity (Shannon index) of gastrointestinal microbiota. (C). Principal coordinate analysis (PCoA) was used to investigate the similarity of microbial communities at different time points. (D). Strains with the same change trend before and after treatment with three treatments. (E). Correlation between strains with the same change trend and metabolites in neg mode. *p < 0.05.
Figure 4
Figure 4
Characteristics of gastrointestinal microbiota in patient case 2. (A). Composition of the top 10 species at the species level. (B). The diversity (Shannon index) of gastrointestinal microbiota. (C). Principal coordinate analysis (PCoA) was used to investigate the similarity of microbial communities at different time points. (D). Strains with the same change trend before and after treatment with three treatments. (E). Correlation between strains with the same change trend and metabolites in pos mode. (F). Correlation between strains with the same change trend and metabolites in neg mode. *p < 0.05.
Figure 5
Figure 5
Variation trend of Eubacterium eligens before and after different treatment. (A) Case 1, (B) Case 2.
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