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. 2023 Jan;12(3):e2201925.
doi: 10.1002/adhm.202201925. Epub 2022 Oct 31.

Swelling-Mediated Mechanical Stimulation Regulates Differentiation of Adipose-Derived Mesenchymal Stem Cells for Intervertebral Disc Repair Using Injectable UCST Microgels

Xianpeng Huang  1   2   3   4 , Di Chen  5 , Chengzhen Liang  1   2   3   4 , Kesi Shi  1   2   3   4 , Xiaopeng Zhou  1   2   3   4 , Yuang Zhang  1   2   3   4 , Yi Li  1   2   3   4 , Jiangjie Chen  1   2   3   4 , Kaishun Xia  1   2   3   4 , Jiawei Shu  1   2   3   4 , Biao Yang  1   2   3   4 , Jingkai Wang  1   2   3   4 , Haibin Xu  1   2   3   4 , Chao Yu  1   2   3   4 , Feng Cheng  1   2   3   4 , Shaoke Wang  1   2   3   4 , Yongxiang Zhang  1   2   3   4 , Chenggui Wang  6 , Liwei Ying  1   2   3   4 , Hao Li  1   2   3   4 , Meiling Han  7 , Fangcai Li  1   2   3   4 , Yiqing Tao  1   2   3   4 , Qian Zhao  8 , Qixin Chen  1   2   3   4
Affiliations

Swelling-Mediated Mechanical Stimulation Regulates Differentiation of Adipose-Derived Mesenchymal Stem Cells for Intervertebral Disc Repair Using Injectable UCST Microgels

Xianpeng Huang et al. Adv Healthc Mater. 2023 Jan.

Abstract

Mechanical stimulation is an effective approach for controlling stem cell differentiation in tissue engineering. However, its realization in in vivo tissue repair remains challenging since this type of stimulation can hardly be applied to injectable seeding systems. Here, it is presented that swelling of injectable microgels can be transformed to in situ mechanical stimulation via stretching the cells adhered on their surface. Poly(acrylamide-co-acrylic acid) microgels with the upper critical solution temperature property are fabricated using inverse emulsion polymerization and further coated with polydopamine to increase cell adhesion. Adipose-derived mesenchymal stem cells (ADSCs) adhered on the microgels can be omnidirectionally stretched along with the responsive swelling of the microgels, which upregulate TRPV4 and Piezo1 channel proteins and enhance nucleus pulposus (NP)-like differentiation of ADSCs. In vivo experiments reveal that the disc height and extracellular matrix content of NP are promoted after the implantation with the microgels. The findings indicate that swelling-induced mechanical stimulation has great potential for regulating stem cell differentiation during intervertebral disc repair.

Keywords: injectable upper critical temperature microgels; intervertebral disc repairs; mechanical stimulations; stem cell differentiations.

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