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. 2023:2589:17-25.
doi: 10.1007/978-1-0716-2788-4_2.

Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells

Miriam Pons  1 , Mandy Beyer  2
Affiliations

Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells

Miriam Pons et al. Methods Mol Biol. 2023.

Abstract

One of the main characteristics of cancer is the rapid proliferation of transformed cells. Cancer therapies aim to kill such cells. Cancer clones surviving therapy can be resistant to the treatment, but they can also lose the ability to proliferate. The ability of single cells to proliferate can be monitored in vitro and can provide insights into the sensitivity of tumor cells to chemotherapeutics. The following chapter describes how clonogenic hematopoietic cell growth can be determined with the colony formation assay.

Keywords: Colony formation; HDAC; Hematopoietic cells; Leukemia; Methyl cellulose; Proliferation; Survival.

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