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. 2023 Jan;29(1):483-486.
doi: 10.1111/cns.13995. Epub 2022 Oct 18.

A marked enhancement of a BLOC-1 gene, pallidin, associated with somnolent mouse models deficient in histamine transmission

Laurent Seugnet  1 , Christelle Anaclet  1   2 , Magali Perier  1 , Jean-François Ghersi-Egea  3 , Jian-Sheng Lin  1
Affiliations

A marked enhancement of a BLOC-1 gene, pallidin, associated with somnolent mouse models deficient in histamine transmission

Laurent Seugnet et al. CNS Neurosci Ther. 2023 Jan.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Dr. Lin is an Editorial Board member of CNS Neuroscience and Therapeutics and a coauthor of this article. To exclude any bias, he was not involved in any editorial decision‐making related to the acceptance of this article for publication.

Figures

FIGURE 1
FIGURE 1
(A) Selection of differentially expressed genes in HO‐KO mice, confirmed by QPCR. Relative expression as % of controls is shown (N = 3–7, p < 0.05). (B) Relative expression levels as % of controls in the hypothalamus (top row), thalamus (middle row), and frontal cortex (bottom row) of HO‐KO mice, for the 3′ untranslated part of the truncated transcripts of Histidine decarboxylase (Hdc) and Orexin in the KO. Right: expression of pallidin in the same brain regions. N = 4–8. (C) Relative expression levels of pallidin transcripts in the frontal cortex HO‐KO compared to Hdc, Histamine H1 Receptor (H1R), and Orexin (Ox) single KO mice. N = 4–8. (D) Left: levels of Noradrenaline (NA), Dopamine (DA), and Serotonin (5‐HT) as measured by HPLC in the frontal cortex of HO‐KO mice, right: relative transcripts levels for the tyrosine hydroxylase (TH), Choline acetyltransferase (ChAT), and Muscarinic acetylcholine receptor M4 (M4) genes in the frontal cortex of HO‐KO mice (N = 4–9). (E) Relative expression levels of pallidin transcripts in the choroid plexuses of Histidine decarboxylase single KO mice. (F, G) Overexpression of pallidin in Drosophila. The experimental flies are in pink; the genetic controls are in blue. N = 20–21. (F) Overexpression of pallidin in neurons, the elav‐GeneSwitch construct was used as Gal4. (G) Overexpression of pallidin in all glial cells using the Gal4‐UAS system. The Repo‐Gal4 transgene was combined with the Tub‐Gal80ts to inhibit Gal4 activity at 18°C. Flies were transferred at 30°C to induce the expression of pallidin. Left: total daily sleep, right: hourly sleep curve of the first 24 h spent at 30°C. *p < 0.05; **p < 0.005; ***p < 0.0005. See Appendix S1 for additional details and statistics.
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References

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