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. 2022 Nov;23(6):e82.
doi: 10.4142/jvs.22178. Epub 2022 Sep 15.

Comparative in vitro biotransformation of fipronil in domestic poultry using liver microsome

Kraisiri Khidkhan  1 , Saranya Poapolathep  2 , Sittinee Kulprasertsri  3 , Rattapong Sukkheewan  2 , Paphatsara Khunlert  4 , Mario Giorgi  5 , Amnart Poapolathep  6
Affiliations

Comparative in vitro biotransformation of fipronil in domestic poultry using liver microsome

Kraisiri Khidkhan et al. J Vet Sci. 2022 Nov.

Abstract

Domestic poultry are among the non-target species of exposure to fipronil, but limited information is available on the metabolic effects of fipronil exposure in avian. We investigated the comparative capacity of in vitro biotransformation of fipronil among chicken, duck, quail, goose, and rat. Interspecies differences in kinetic parameters were observed; the clearance rate calculations (Vmax/Km) indicated that chicken and duck are more efficient in the cytochrome P450-mediated metabolism of fipronil to sulfone than quail, goose and rat. The lower hepatic clearance of fipronil in quail, goose and rat, suggested that fipronil sulfone may serve as a biomarker to indicate fipronil exposure in these species.

Keywords: Biotransformation; cytochrome P450; microsome; poultry; sulfone.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1. Michaelis-Menten plots of chickens, ducks, quails, and rats obtained from the reactions for metabolic activity using their microsomal fractions (mean ± SD).
See this image and copyright information in PMC

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