Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Oct 3:10:961987.
doi: 10.3389/fbioe.2022.961987. eCollection 2022.

General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications

Michela Pozzobon  1 , Stefania D'Agostino  1 , Maria G Roubelakis  2 , Anna Cargnoni  3 , Roberto Gramignoli  4 , Susanne Wolbank  5 , Florelle Gindraux  6 , Sveva Bollini  7 , Halima Kerdjoudj  8 , Mathilde Fenelon  9 , Roberta Di Pietro  10 , Mariangela Basile  10 , Veronika Borutinskaitė  11 , Roberta Piva  12 , Andreina Schoeberlein  13 , Guenther Eissner  14 , Bernd Giebel  15 , Peter Ponsaerts  16
Affiliations
Review

General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications

Michela Pozzobon et al. Front Bioeng Biotechnol. .

Abstract

Perinatal tissues, such as placenta and umbilical cord contain a variety of somatic stem cell types, spanning from the largely used hematopoietic stem and progenitor cells to the most recently described broadly multipotent epithelial and stromal cells. As perinatal derivatives (PnD), several of these cell types and related products provide an interesting regenerative potential for a variety of diseases. Within COST SPRINT Action, we continue our review series, revising and summarizing the modalities of action and proposed medical approaches using PnD products: cells, secretome, extracellular vesicles, and decellularized tissues. Focusing on the brain, bone, skeletal muscle, heart, intestinal, liver, and lung pathologies, we discuss the importance of potency testing in validating PnD therapeutics, and critically evaluate the concept of PnD application in the field of tissue regeneration. Hereby we aim to shed light on the actual therapeutic properties of PnD, with an open eye for future clinical application. This review is part of a quadrinomial series on functional/potency assays for validation of PnD, spanning biological functions, such as immunomodulation, anti-microbial/anti-cancer, anti-inflammation, wound healing, angiogenesis, and regeneration.

Keywords: amniotic membrane and fluid stem cells; extracellular vesicles; perinatal derivatives; regenerative medicine; tissue regeneration.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Flow chart of the manuscript organization.
FIGURE 2
FIGURE 2
Schematic representation of the use of different PnD (tissues, cells, and paracrine derivatives—secretome (whole soluble factors including EVs) and isolated EVs), their application in different diseased organs and the functional assays. Created with BioRender.com.
See this image and copyright information in PMC

References

    1. Abreu S. C., Weiss D. J., Rocco P. R. M. (2016). Extracellular vesicles derived from mesenchymal stromal cells: A therapeutic option in respiratory diseases? Stem Cell Res. Ther. 7 (1), 53. 10.1186/s13287-016-0317-0 - DOI - PMC - PubMed
    1. Arutyunyan I., Fatkhudinov T., Elchaninov A., Vasyukova O., Makarov A., Usman N., et al. (2019). Umbilical cord-derived mesenchymal stromal/stem cells enhance recovery of surgically induced skeletal muscle ischemia in a rat model. Histol. Histopathol. 34 (5), 513–523. 10.14670/HH-18-057 - DOI - PubMed
    1. Balbi C., Costa A., Barile L., Bollini S. (2020). Message in a bottle: Upgrading cardiac repair into rejuvenation. Cells 9 (3), 724. 10.3390/cells9030724 - DOI - PMC - PubMed
    1. Balbi C., Lodder K., Costa A., Moimas S., Moccia F., van Herwaarden T., et al. (2019). Reactivating endogenous mechanisms of cardiac regeneration via paracrine boosting using the human amniotic fluid stem cell secretome. Int. J. Cardiol. 287, 87–95. 10.1016/j.ijcard.2019.04.011 - DOI - PubMed
    1. Balbi C., Lodder K., Costa A., Moimas S., Moccia F., van Herwaarden T., et al. (2019). Supporting data on in vitro cardioprotective and proliferative paracrine effects by the human amniotic fluid stem cell secretome. Data Brief. 25, 104324. 10.1016/j.dib.2019.104324 - DOI - PMC - PubMed