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. 2022 Dec 16;23(24):e202200595.
doi: 10.1002/cbic.202200595. Epub 2022 Nov 17.

In vitro Applications of the Terpene Mini-Path 2.0

Julie Couillaud  1   2 , Agnès Amouric  1 , Elise Courvoisier-Dezord  1 , Létitia Leydet  1 , Nicolas Schweitzer  1 , Marie-Noëlle Rosso  3 , Hayat Hage  3 , Margot Loussouarn-Yvon  3 , Renaud Vincentelli  4 , Jean-Louis Petit  5 , Véronique de Berardinis  5 , Mireille Attolini  1 , Marc Maresca  1 , Katia Duquesne  1 , Gilles Iacazio  1
Affiliations

In vitro Applications of the Terpene Mini-Path 2.0

Julie Couillaud et al. Chembiochem. .

Abstract

In 2019 four groups reported independently the development of a simplified enzymatic access to the diphosphates (IPP and DMAPP) of isopentenol and dimethylallyl alcohol (IOH and DMAOH). The former are the two universal precursors of all terpenes. We report here on an improved version of what we call the terpene mini-path as well as its use in enzymatic cascades in combination with various transferases. The goal of this study is to demonstrate the in vitro utility of the TMP in, i) synthesizing various natural terpenes, ii) revealing the product selectivity of an unknown terpene synthase, or iii) generating unnatural cyclobutylated terpenes.

Keywords: (+)-δ-cadinol; cyclobutyl terpenoid derivatives; farnesyl diphosphate; terpene mini-path; tryprostatin B.

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