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. 2023 Feb;43(1):93-103.
doi: 10.1007/s11596-022-2609-1. Epub 2022 Oct 21.

High Level of Ubiquitin Conjugate Enzyme E2O Indicates Poor Prognosis of Patients with Hepatocellular Carcinoma

Si-Yu Lan  1   2 , Yang Ding  1   2 , Chun Wang  1   2 , Jun Fang  1   2 , Chao Ren  1   2 , Jia-Liang Liu  1   2 , Hui Kang  1   2 , Ying Chang  3   4
Affiliations

High Level of Ubiquitin Conjugate Enzyme E2O Indicates Poor Prognosis of Patients with Hepatocellular Carcinoma

Si-Yu Lan et al. Curr Med Sci. 2023 Feb.

Abstract

Objective: Ubiquitin conjugate enzyme E2O (UBE2O) is a ubiquitin-conjugating enzyme that has been reported to be involved in tumorigenesis. This study investigated the role of UBE2O in hepatocellular carcinoma (HCC).

Methods: The expression of UBE2O was detected using qRT-PCR, Western blotting, and immunohistochemical staining. Cell proliferation and Transwell assays were used to detect proliferation, migration, and invasion of HCC cells, respectively. Bioinformatic analysis was performed to analyze the relationship between UBE2O and the clinical features, prognosis, and immune cell infiltration of HCC.

Results: UBE2O was significantly over-expressed in HCC tissues. High expression of UBE2O was associated with poor tumor grade and poor prognosis. Functional experiments showed that down-regulation of UBE2O inhibited HCC cell proliferation, migration, and invasion. Co-expression gene analysis and gene set enrichment analysis showed that UBE2O was associated with protein hydrolysis, cell cycle, and cancer-related pathways in HCC. The results of immune analysis revealed that the expression of UBE2O was positively correlated with the immune infiltration and expression of immune-related chemokines of HCC.

Conclusions: UBE2O is significantly correlated with the prognosis of HCC and may be a valuable prognostic biomarker for HCC.

Keywords: hepatocellular carcinoma; immune; prognosis; ubiquitin conjugate enzyme E2O.

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