Establishing a New Research Axolotl Colony

Abstract

The field of regenerative biology has taken a keen interest in the Mexican axolotl (Ambystoma mexicanum) over the past few decades, as this salamander successfully regenerates amputated limbs and injured body parts. Recent progress in research tool development has also made possible axolotl genetic manipulation and single-cell analysis, which will help understand the molecular mechanisms of complex tissue regeneration. To support the growing popularity of this model, we describe how to set up a new axolotl housing facility at a research laboratory. We also review husbandry practices for raising axolotls and using them in biological research, with a focus on diet, water quality, breeding, and anesthesia.

Keywords: Aquatic; Axolotl; Housing; Husbandry.

Fig. 1

Housing juvenile axolotls. (a) Young axolotls (up to 1 cm in length) can be housed in food-grade plastic dishes and stored on stacked cafeteria trays. (b) Insulation tape on the bottom of trays prevents animals on the tray below from suffocating

Fig. 2

Remote surveillance of an egg-laying female. The female is preparing to spawn in a temperature-controlled incubator, and a tablet is being used to monitor the process during the night