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. 2023:2562:165-173.
doi: 10.1007/978-1-0716-2659-7_10.

Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the Mexican Axolotl

Melissa Keinath  1 , Vladimir Timoshevskiy  2
Affiliations

Fluorescence In Situ Hybridization of DNA Probes on Mitotic Chromosomes of the Mexican Axolotl

Melissa Keinath et al. Methods Mol Biol. 2023.

Abstract

Fluorescence in situ hybridization (FISH) is used extensively for visual localization of specific DNA fragments (and RNA fragments) in broad applications on chromosomes or nuclei at any stage of the cell cycle: metaphase, anaphase, or interphase. The cytogenetic slides that serve as a target for the labeled DNA probe might be prepared using any approach suitable for obtaining cells with appropriate morphology for imaging and analysis. In this chapter, we focus on the application of molecular cytogenetic methods such as DNA labeling, slide preparation, and in situ hybridization related to cells from Mexican axolotl.

Keywords: Axolotl mitotic chromosomes; BAC; Chromosomal in situ suppression (CISS) hybridization; Fluorescence in situ hybridization; Nick translation.

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References

    1. Pardue ML, Gall JG (1969) Molecular hybridization of radioactive DNA to the DNA of cytological preparations. Proc Natl Acad Sci U S A 64(2):600–604 - DOI
    1. Raj A, van den Bogaard P, Rifkin SA, van Oudenaarden A, Tyagi S (2008) Imaging individual mRNA molecules using multiple singly labeled probes. Nat Methods 5(10):877–879 - DOI
    1. Jin L, Lloyd RV (1997) In situ hybridization: methods and applications. J Clin Lab Anal 11(1):2–9 - DOI
    1. Keinath MC, Timoshevskiy VA, Timoshevskaya NY, Tsonis PA, Voss SR, Smith JJ (2015) Initial characterization of the large genome of the salamander Ambystoma mexicanum using shotgun and laser capture chromosome sequencing. Sci Rep 5:16413 - DOI
    1. Schloissnig S, Kawaguchi A, Nowoshilow S, Falcon F, Otsuki L, Tardivo P et al (2021) The giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci. Proc Natl Acad Sci U S A 118(15):e2017176118 - DOI

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