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. 2023:2562:183-194.
doi: 10.1007/978-1-0716-2659-7_12.

COMET Assay for Detection of DNA Damage During Axolotl Tail Regeneration

Belfran Carbonell  1   2 , Jennifer Álvarez  1 , Gloria A Santa-González  1   3 , Jean Paul Delgado  4
Affiliations

COMET Assay for Detection of DNA Damage During Axolotl Tail Regeneration

Belfran Carbonell et al. Methods Mol Biol. 2023.

Abstract

The purpose of this chapter is to evaluate DNA damage during axolotl tail regeneration using an alkaline comet assay. Our method details the isolation of cells from regenerating and non-regenerating tissues and the isolation of peripheral blood for single-cell gel electrophoresis. Also, we detail each of the steps for the development of the comet assay technique which includes mounting the isolated cells on an agarose matrix, alkaline electrophoresis, and DNA damage detection.

Keywords: Alkaline comet assay; DNA damage; Tail regeneration; single-cell gel electrophoresis.

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