Population genetic structure of fringe-lipped carp, Labeo fimbriatus from the peninsular rivers of India

Abstract

Labeo fimbriatus is a medium carp species found throughout India's peninsular river basins and is regarded as a valuable aquaculture resource alongside Indian major carps due to its taste and nutritional value. This species has recently declined dramatically due to habitat degradation and overfishing. Because of its enormous economic importance, a selective breeding programme is likely to be in place to improve performance traits. Knowledge of genetic variation among the base population from which the broodstock will be selected is an important step in this process. A diverse genetic base of broodstock is required to achieve the best response to selection for long-term aquaculture management practices. Consequently, using mitochondrial DNA (ATPase 6 and Control region) and microsatellite markers, we have made the first step toward estimating the level of genetic variation and how it is distributed among the four populations of L. fimbriatus found in peninsular rivers in India. The ATPase 6 gene analysis in four populations revealed 15 haplotypes and 51 variable sites, in contrast to the Control region, which had 60 haplotypes together with 73 variable sites and a haplotype diversity of 0.941. Twelve microsatellite loci displayed estimated allele numbers (N _A) ranging from 3 to 19, observed heterozygosity (H _O), and expected heterozygosity (H _E), respectively, of 0.705 to 0.753 and 0.657 to 0.914. Each marker type showed a significant F _ST value, indicating the presence of low to moderate genetic differentiation across entire wild populations. The Godavari, Kaveri, and Mahanadi populations formed one cluster according to the UPGMA, which was based on genetic distance matrix, while the Krishna population formed a separate cluster. The comparative genetic analysis of data from different markers utilized in the current study would enable the identification of the genetic stocks of L. fimbriatus and facilitate conservation measures and selective breeding.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-022-03369-y.

Keywords: Genetic diversity; Labeo fimbriatus; Microsatellite; Population structure; mtDNA.

Fig. 1

Partial map of India showing the rivers from where the samples were collected

Fig. 2

UPGMA dendrogram based on genetic distance among four wild populations of L. fimbriatus

Fig. 3

Bar plots from STRUCTURE suggesting four clusters (K = 5) based on 12 polymorphic microsatellite loci