Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec;62(12):2609-2620.
doi: 10.1111/trf.17160. Epub 2022 Oct 24.

Donor variation in stored platelets: Higher metabolic rates of platelets are associated with mean platelet volume, activation and donor health

Ido J Bontekoe  1 , Pieter F van der Meer  1   2 , Bea C Tanis  3 , Dirk de Korte  1   4 , Arthur J Verhoeven  5 , Nicolaas J H Raat  6 , Patricia A C Specht  6 , Egbert G Mik  6 , Thomas R L Klei  1
Affiliations

Donor variation in stored platelets: Higher metabolic rates of platelets are associated with mean platelet volume, activation and donor health

Ido J Bontekoe et al. Transfusion. 2022 Dec.

Abstract

Background: Platelets (PLTs) differ in glycolytic activity, resulting in rapid acidification of 'poor' storing PLT concentrates (PCs) in plasma, or depletion of glucose when stored in PLT additive solution (PAS). We aimed to understand why PLT glycolysis rates vary between donors and how this affects storage performance.

Study design and methods: Buffy coats from donors <45, 45-70 and >70 years were selected and single-donor PCs in plasma or PAS-E were prepared. PCs were stored for 8 days at 22 ± 2°C and sampled regularly for analysis. Mitochondrial activity was analyzed with an Oroboros oxygraph. Age groups, or subgroups divided into quartiles based on glucose consumption, were analyzed with ANOVA.

Results: In each comparison, PCs of the different groups were not different in volume and cellular composition. PLTs with the highest glucose consumption had a higher initial mean platelet volume (MPV) and developed higher CD62P expression and Annexin A5 binding during storage. Higher glycolytic activity in these PLTs was not a compensation for lower mitochondrial ATP production, because mitochondrial ATP-linked respiration of fresh PLTs correlated positively with MPV (R2 = 0.71). Donors of high glucose-consuming PLTs had more health-related issues. Storage properties of PCs from donors over 70 were not significantly different compared to PCs from donors younger than 45 years.

Conclusions: High glucose-consuming PCs developing higher activation levels, not only displayed enhanced mitochondrial activity but were also found to contain larger PLTs, as determined by MPV. Storage performance of PLTs was found to be associated with donor health, but not with donor age.

Keywords: blood component preparations; donors; platelet transfusion.

PubMed Disclaimer

References

REFERENCES

    1. Bontekoe IJ, van der Meer PF, van den Hurk K, Verhoeven AJ, de Korte D. Platelet storage performance is consistent by donor: a pilot study comparing "good" and "poor" storing platelets. Transfusion. 2017;57:2373-80.
    1. Bontekoe IJ, van der Meer PF, Verhoeven AJ, et al. Platelet storage properties are associated with donor age: in vitro quality of platelets from young donors and older donors with and without Type 2 diabetes. Vox Sang. 2019;114:129-36.
    1. Bontekoe IJ, van der Meer PF, de Korte D. Determination of thromboelastographic responsiveness in stored single-donor platelet concentrates. Transfusion. 2014;54:1610-8.
    1. Sjövall F, Ehinger JKH, Marelsson SE, Morota S, Åsander Frostner E, Uchino H, et al. Mitochondrial respiration in human viable platelets-methodology and influence of gender, age and storage. Mitochondrion. 2013;13:7-14.
    1. Aibibula M, Naseemi KM, Sturmey RG. Glucose metabolism and metabolic flexibility in blood platelets. J Thromb Haemost. 2018;16:2300-14.

Substances

LinkOut - more resources