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. 2022 Dec;175(12):1771-1774.
doi: 10.7326/M22-2089. Epub 2022 Oct 25.

Safety and Immunogenicity of Intradermal Fractional Dose Administration of the mRNA-1273 Vaccine: A Proof-of-Concept Study

Geert V T Roozen  1 , Margaretha L M Prins  1 , Rob van Binnendijk  2 , Gerco den Hartog  2 , Vincent P Kuiper  3 , Corine Prins  1 , Jacqueline J Janse  3 , Annelieke C Kruithof  4 , Mariet C W Feltkamp  5 , Marjan Kuijer  2 , Frits R Rosendaal  6 , Meta Roestenberg  7 , Leo G Visser  1 , Anna H E Roukens  1
Affiliations

Safety and Immunogenicity of Intradermal Fractional Dose Administration of the mRNA-1273 Vaccine: A Proof-of-Concept Study

Geert V T Roozen et al. Ann Intern Med. 2022 Dec.
No abstract available

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Conflict of interest statement

Disclosures: Disclosures can be viewed at www.acponline.org/authors/icmje/ConflictOfInterestForms.do?msNum=M22-2089.

Figures

Figure.
Figure.. Serum anti–SARS-CoV-2 antibody concentrations.
Each symbol represents a sample from an individual participant at a certain time point. Error bars represent geometric mean concentrations with 95% CIs. anti-N = anti-nucleocapsid; anti-RBD = anti–receptor-binding domain; anti-S1 = anti–spike S1; AU = arbitrary unit; BAU = binding antibody unit; ID = intradermal; IM = intramuscular; LLoQ = lower limit of quantitation; NF50 = neutralizing factor at 50% normalized against an international reference serum. A, B, and C. Anti–SARS-CoV-2 serum IgG concentrations for anti-S1 (A), anti-RBD (B), and anti-N (C), assessed by bead-based immunoassay and reported in BAUs per milliliter. Data at day 1 (before receipt of the first vaccination), day 29 (before receipt of the second vaccination), day 36 (1 week after receipt of the second vaccination), day 43 (2 weeks after receipt of the second vaccination), and month 7 (half a year after receipt of the second vaccination) are reported for the 10-mcg ID dose, the 20-mcg ID dose, and the 20-mcg IM dose. Horizontal dashed lines represent the cutoff for seropositivity (10 BAU/mL for anti-S1, 30 BAU/mL for anti-RBD, and 14 BAU/mL for anti-N). D. Wild-type SARS-CoV-2 (Wuhan strain) microneutralization assay. NF50 is reported in international units per milliliter. The conversion rate from titer to international units was 1 / 4.064. The horizontal dashed line represents the LLoQ at 15 IU/mL (titer of 62). Values with an NF50 below the LLoQ were set to 2 IU/mL (titer of 10). Values above the upper limit of quantitation (n = 2 in the 20-mcg ID group and 1 in the 20-mcg IM group at day 43) were set to 2953 IU/mL (titer of 12 000). E, F, and G. Anti–SARS-CoV-2 serum IgA concentrations for anti-S1 (E), anti-RBD (F), and anti-N (G), assessed by bead-based immunoassay and reported in AUs per milliliter. Data at days 1, 29, 36, and 43 are reported for the 10-mcg ID dose, the 20-mcg ID dose, and the 20-mcg IM dose.
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References

    1. Roozen GVT , Roukens AHE , Roestenberg M . COVID-19 vaccine dose sparing: strategies to improve vaccine equity and pandemic preparedness. Lancet Glob Health. 2022;10:e570-e573. [PMID: ] doi:10.1016/S2214-109X(22)00075-4 - DOI - PMC - PubMed
    1. Nicolas JF , Guy B . Intradermal, epidermal and transcutaneous vaccination: from immunology to clinical practice. Expert Rev Vaccines. 2008;7:1201-14. [PMID: ] doi:10.1586/14760584.7.8.1201 - DOI - PubMed
    1. Gilbert PB , Montefiori DC , McDermott AB , et al; Immune Assays Team. Immune correlates analysis of the mRNA-1273 COVID-19 vaccine efficacy clinical trial. Science. 2022;375:43-50. [PMID: ] doi:10.1126/science.abm3425 - DOI - PMC - PubMed

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