Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023 Feb;164(3):270-283.
doi: 10.1111/jnc.15711. Epub 2022 Nov 5.

New imaging instrument in animal models: Two-photon miniature microscope and large field of view miniature microscope for freely behaving animals

Changliang Guo  1   2 , Aimin Wang  3   4 , Heping Cheng  2   5 , Liangyi Chen  2   6   7
Affiliations
Review

New imaging instrument in animal models: Two-photon miniature microscope and large field of view miniature microscope for freely behaving animals

Changliang Guo et al. J Neurochem. 2023 Feb.

Abstract

Over the past decade, novel optical imaging tools have been developed for imaging neuronal activities along with the evolution of fluorescence indicators with brighter expression and higher sensitivity. Miniature microscopes, as revolutionary approaches, enable the imaging of large populations of neuron ensembles in freely behaving rodents and mammals, which allows exploring the neural basis of behaviors. Recent progress in two-photon miniature microscopes and mesoscale single-photon miniature microscopes further expand those affordable methods to navigate neural activities during naturalistic behaviors. In this review article, two-photon miniature microscopy techniques are summarized historically from the first documented attempt to the latest ones, and comparisons are made. The driving force behind and their potential for neuroscientific inquiries are also discussed. Current progress in terms of the mesoscale, i.e., the large field-of-view miniature microscopy technique, is addressed as well. Then, pipelines for registering single cells from the data of two-photon and large field-of-view miniature microscopes are discussed. Finally, we present the potential evolution of the techniques.

Keywords: large field-of-view imaging; miniature fluorescence microscopy; neural imaging; neuroscience; two-photon microscopy.

PubMed Disclaimer

References

REFERENCES

    1. Adam, Y., Kim, J. J., Lou, S., Zhao, Y., Xie, M. E., Brinks, D., Wu, H., Mostajo-Radji, M. A., Kheifets, S., Parot, V., Chettih, S., Williams, K. J., Gmeiner, B., Farhi, S. L., Madisen, L., Buchanan, E. K., Kinsella, I., Zhou, D., Paninski, L., … Cohen, A. E. (2019). Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics. Nature, 569(7756), 413-417. https://doi.org/10.1038/s41586-019-1166-7
    1. Adesnik, H., & Abdeladim, L. (2021). Probing neural codes with two-photon holographic optogenetics. Nature Neuroscience, 24(10), 1356-1366. https://doi.org/10.1038/s41593-021-00902-9
    1. Aghajan, Z. M., Acharya, L., Moore, J. J., Cushman, J. D., Vuong, C., & Mehta, M. R. (2015). Impaired spatial selectivity and intact phase precession in two-dimensional virtual reality. Nature Neuroscience, 18(1), 121-128. https://doi.org/10.1038/nn.3884
    1. Aharoni, D., & Hoogland, T. M. (2019). Circuit investigations with open-source miniaturized microscopes: Past, present and future. Frontiers in Cellular Neuroscience, 13, 141. https://doi.org/10.3389/fncel.2019.00141
    1. Aharoni, D., Khakh, B. S., Silva, A. J., & Golshani, P. (2019). All the light that we can see: A new era in miniaturized microscopy. Nature Methods, 16, 11-13. https://doi.org/10.1038/s41592-018-0266-x

Publication types

LinkOut - more resources