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. 2023 Feb;9(1):112-121.
doi: 10.1002/cre2.678. Epub 2022 Oct 25.

Interactive role of miR-29, miR-93, miR-205, and VEGF in salivary adenoid cystic carcinoma

Parisa Bayat  1 , Nazanin Mahdavi  2 , Shima Younespour  1 , Neda Kardouni Khoozestani  2   3
Affiliations

Interactive role of miR-29, miR-93, miR-205, and VEGF in salivary adenoid cystic carcinoma

Parisa Bayat et al. Clin Exp Dent Res. 2023 Feb.

Abstract

Objectives: Salivary adenoid cystic carcinoma (SACC) is one of the most common salivary gland tumors in which patients encounter local recurrence and lung metastases. Understanding prognostic biomarkers in SACC is essential for future development in prognosis and treatment. This study aimed to assess the expression level of vascular endothelial growth factor (VEGF) and its potential regulatory microRNAs in SACC for prognostic determination. MATERIAL AND METHODS: The expression of VEGF in SACC samples was assessed using immunohistochemistry. Potential regulatory microRNAs were evaluated using quantitative reverse transcription-polymerase chain reaction. Associations between VEGF and microRNAs expression and clinicopathological parameters were investigated.

Results: VEGF expression levels positively correlated with histologic grade (p = .004) and treatment modality (p = .04). Decreased expression of miR-29a (p = .01) and increased expression of miR-93-5p and miR-205 (both p < .0001) were observed in SACC compared to normal salivary gland tissue. MiR-93-5p showed a positive association (p = .02) with VEGF overexpression.

Conclusions: Our results showed the downregulation of miR-29 and overexpression of miR-93 and miR-205 in the SACC group, and the correlation between miR-93 and VEGF suggests these biomarkers as potential prognostic markers in the future.

Keywords: miR-205; miR-29; miR-93; salivary adenoid cystic carcinoma.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Vascular endothelial growth factor immunohistochemistry staining of SACC in normal serous salivary gland tissue (a), in inflammatory cells infiltration of an inflamed specimen (b), SACC cribriform pattern (c, d), SACC solid pattern (e, f). SACC, salivary adenoid cystic carcinoma.
Figure 2
Figure 2
Relative expression levels of (a) VEGF, (b) miR‐29a, (c) miR‐93‐5p, and (d) miR‐205, Middle point: median; box: interquartile range (25th to 75th percentiles); whisker: range (excluding outliers). Each error bar is constructed using a 95% confidence interval of the mean. miR, microRNA; SACC, salivary adenoid cystic carcinoma; VEGF, vascular endothelial growth factor.
See this image and copyright information in PMC

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