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. 2022 Oct 25;18(1):378.
doi: 10.1186/s12917-022-03473-4.

Laparoscopy assisted abomasal cannulation in cadavers of bovine fetuses

Heytor Jales Gurgel  1 , Francisco Décio de Oliveira Monteiro  2   3 , João Pedro Monteiro Barroso  1 , Loise Araújo de Sousa  1 , Gabriela Melo Alves Dos Santos  1 , Kayan da Cunha Rossy  1 , Verena Siqueira da Silva  1 , Camila do Espirito Santo Fernandes  1 , Carla Rozilene Guimarães Silva  1 , Rodrigo Dos Santos Albuquerque  1 , Luisa Pucci Bueno Borges  1 , Luiz Henrique Vilela Araújo  1 , Daniele Lira Dos Santos  1 , Felipe Farias Pereira da Câmara Barros  4 , Pedro Paulo Maia Teixeira  1
Affiliations

Laparoscopy assisted abomasal cannulation in cadavers of bovine fetuses

Heytor Jales Gurgel et al. BMC Vet Res. .

Abstract

Background: Due to the complexity of ruminant digestion, cannulation of organs of the digestive tract has been carried out in order to advance the understanding of digestive physiology, nutrient degradability, gastrointestinal diseases and biotechnological research. The abomasal cannulation is interesting for nutritional studies, especially in suckling calves, to obtain fluid and abomasal content, evaluation of abomasal flow and function, and infusion of nutrients and drugs when it is intended to reach high concentrations in the organ. Conventionally, access and cannulation of digestive organs of ruminants has been performed by laparotomy, a method often criticized and classified as cruel by some sectors related to ethics and animal welfare. The aim of this present study is to describe and standardize a minimally invasive by laparoscopy assisted abomasal cannulation in bovine fetuses (cadavers), which had been previously slaughtered by accident and would be discarded in local slaughterhouses.

Results: The abomasal cannulation technique was feasible, simple and did not present major difficulties. The surgical time for cannulation of the abomasum, from the insertion of the trocars to the completion of the technique with fixation of the organ to the abdominal wall, ranged from 9 to 27 min, with an average of 15.5 ± 6.62 min.

Conclusions: The Laproscopic assisted abomasal cannulation in bovine fetuses was feasible and safe with minimal tissue injury to the abdominal wall and with short surgical time. More studies in the clinical routine related to minimally invasive abomasal content collection, abomasopexy and abomasotomy are required in order to demonstrate its impact and importance in bovine clinic.

Keywords: Abomasum; Animal experimentation; Fistulation; Laparoscopy; Ruminants.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Video-assisted laparoscopic abomasostomy in bovine fetal cadavers. Arrangement of the portals on the right flank of a fetus in left lateral decubitus, first trocar through which the laparoscope is inserted and the CO2 hose is attached (1st), second trocar for insertion of the babcock forceps (2nd), right flank (x) and ribs (y). Cranial (Cr) and Caudal (Cr)
Fig. 2
Fig. 2
Intra-abdominal view with visualization of the abomasum and babcock forceps (1)
Fig. 3
Fig. 3
Intra-abdominal view of the abomasum after abomasostomy, fixation of the abomasal wall, region of greater curvature, to the abdominal wall
Fig. 4
Fig. 4
Final position of the Foley catheter at the end of the procedure
Fig. 5
Fig. 5
Scatter plot representing surgical time vs procedure in each fetus undergoing video-assisted laparoscopic abomasostomy, from the first to the last procedure. Blue line showing the tendency to reduce surgical time due to the learning curve
See this image and copyright information in PMC

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