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. 2023 Jan;252(1):145-155.
doi: 10.1002/dvdy.547. Epub 2022 Nov 28.

Pals1a and aPKCλ are not essential for Schwann cell migration, division or myelination in zebrafish

Marie-José Boueid  1 , Aya Mikdache  1 , Emilie Lesport  1 , Brigitte Delespierre  1 , Julien Loisel-Duwattez  1 , Cindy Degerny  1 , Marcel Tawk  1
Affiliations
Free article

Pals1a and aPKCλ are not essential for Schwann cell migration, division or myelination in zebrafish

Marie-José Boueid et al. Dev Dyn. 2023 Jan.
Free article

Abstract

Background: Schwann cells (SCs) are specialized glial cells of the peripheral nervous system that produce myelin and promote fast action potential propagation. In order to myelinate, SCs engage in a series of events that include migration and division along axons, followed by extensive cytoskeletal rearrangements that ensure axonal ensheathment and myelination. SCs are polarized and extend their processes along an abaxonal-adaxonal axis. Here, we investigate the role of the apical polarity proteins, Pals1a, and aPKCλ, in SC behavior during zebrafish development.

Results: We analyzed zebrafish nok and has mutants deficient for pals1a and aPKCλ function respectively. Using live imaging, transmission electron microscopy and whole mount immunostaining, we show that SCs can migrate and divide appropriately, exhibit normal radial sorting, express myelin markers and ensheath axons on time in has and nok mutants.

Conclusions: Pals1a and aPKCλ are not essential for SC migration, division or myelination in zebrafish.

Keywords: Schwann cell; aPKC; myelin; pals1; peripheral nervous system; polarity; zebrafish.

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References

REFERENCES

    1. Cunningham RL, Herbert AL, Harty BL, Ackerman SD, Monk KR. Mutations in dock1 disrupt early Schwann cell development. Neural Dev. 2018;13(1):17. doi:10.1186/s13064-018-0114-9
    1. Lyons DA, Pogoda HM, Voas MG, et al. erbb3 and erbb2 are essential for schwann cell migration and myelination in zebrafish. Curr Biol. 2005;15(6):513-524. doi:10.1016/j.cub.2005.02.030
    1. Mikdache A, Boueid MJ, Lesport E, et al. Timely Schwann cell division drives peripheral myelination in vivo via Laminin/cAMP pathway. Development. 2022;149(17):dev200640. doi:10.1242/dev.200640
    1. Mikdache A, Fontenas L, Albadri S, et al. Elmo1 function, linked to Rac1 activity, regulates peripheral neuronal numbers and myelination in zebrafish. Cell Mol Life Sci. 2020;77(1):161-177. doi:10.1007/s00018-019-03167-5
    1. Perlin JR, Lush ME, Stephens WZ, Piotrowski T, Talbot WS. Neuronal Neuregulin 1 type III directs Schwann cell migration. Development. 2011;138(21):4639-4648. doi:10.1242/dev.068072

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