Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Sep 28;14(5):765-771.
doi: 10.3390/idr14050078.

Study of Dengue Virus Transovarial Transmission in Aedes spp. in Ternate City Using Streptavidin-Biotin-Peroxidase Complex Immunohistochemistry

Nia Kurnia  1   2 , Yance Kaitana  1 , Christina Leta Salaki  1 , Lucia Cecilia Mandey  1 , Josef Sem Berth Tuda  3 , Trina Ekawati Tallei  4
Affiliations

Study of Dengue Virus Transovarial Transmission in Aedes spp. in Ternate City Using Streptavidin-Biotin-Peroxidase Complex Immunohistochemistry

Nia Kurnia et al. Infect Dis Rep. .

Abstract

Aedes aegypti is the most dominant vector in the transmission of dengue hemorrhagic fever (DHF). In addition to Ae. aegypti, Ae. albopictus is a secondary vector of the dengue virus, and both species are widespread in Indonesia. The dengue virus is transmitted from person to person through the bite of an Aedes spp. The vertical (transovarial) transmission of the dengue virus from infective female mosquitoes to their offspring is one of the means by which the dengue virus maintains its existence in nature. Transovarial dengue virus transmission in Aedes spp. mosquitoes contributes to the spread and maintenance of the dengue epidemic. This study employed a qualitative survey to detect dengue virus transovarial transmission in Ternate using the streptavidin-biotin-peroxidase complex (ISBPC) immunohistochemical test. The ISBPC examination of samples collected from the four subdistricts in Ternate revealed a positive result for transovarial transmission of dengue virus. Four Aedes spp., including two Ae. aegypti females, one Ae. albopictus female, and one Ae. albopictus male, tested positive for transovarial transmission of dengue virus in the district of North Ternate. Four Aedes spp., including three Ae. aegypti females and one Ae. aegypti male, were found to be positive for the transovarial transmission of dengue virus in the Central Ternate district. Seven Aedes spp., including five Ae. aegypti females, one Ae. aegypti male, and one Ae. albopictus female, tested positive for transovarial transmission of the dengue virus in the district of South Ternate city. One Ae. aegypti male showed positive results for transovarial transmission of dengue virus in the Ternate Island District. In this study, the transovarial transmission of the dengue virus occurred in both Aedes spp. female and male mosquitoes. It was demonstrated that Aedes spp. carry the dengue virus in their ovaries and can pass it on to their offspring. As a result, the cycle of passing the dengue virus on to local mosquito populations in the city of Ternate is not going to end just yet.

Keywords: Ternate; dengue virus; immunochemistry; transovarial transmission.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The results of microscopic preparations of the ISBPC assay: (a) positive control; (b) negative control; (c) positive result of Ae. Aegypti; (d) negative result of Ae. Albopictus. The presence of antigen was indicated by the brown color of the granules and cytoplasm of hemocytes from head squash preparations. A negative result is indicated by the absence of visible brown or blue color.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Murugesan A., Manoharan M. Dengue Virus. Emerg. Reemerg. Viral Pathogens. 2020;1:281–359.
    1. Murray N.E.A., Quam M.B., Wilder-Smith A. Epidemiology of Dengue: Past, Present and Future Prospects. Clin. Epidemiol. 2013;5:299–309. doi: 10.2147/CLEP.S34440. - DOI - PMC - PubMed
    1. Boulaaras S., Jan R., Khan A., Ahsan M. Dynamical Analysis of the Transmission of Dengue Fever via Caputo-Fabrizio Fractional Derivative. Chaos Solitons Fractals X. 2022;8:100072. doi: 10.1016/j.csfx.2022.100072. - DOI
    1. White A.V., Fan M., Mazzara J.M., Roper R.L., Richards S.L. Mosquito-Infecting Virus Espirito Santo Virus Inhibits Replication and Spread of Dengue Virus. J. Med. Virol. 2021;93:3362–3373. doi: 10.1002/jmv.26686. - DOI - PubMed
    1. Rosen L., Shroyer D.A., Tesh R.B., Freier J.E., Lien J.C. Transovarial Transmission of Dengue Viruses by Mosquitoes: Aedes Albopictus and Aedes Aegypti. Am. J. Trop. Med. Hyg. 1983;32:1108–1119. doi: 10.4269/ajtmh.1983.32.1108. - DOI - PubMed