Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Nov 15;66(11):e0064822.
doi: 10.1128/aac.00648-22. Epub 2022 Oct 26.

Emergence of KPC-31, a KPC-3 Variant Associated with Ceftazidime-Avibactam Resistance, in an Extensively Drug-Resistant ST235 Pseudomonas aeruginosa Clinical Isolate

Diego Faccone  1   2 , Juan M de Mendieta  1 , Ezequiel Albornoz  1 , Magali Chavez  1 , Fabiana Genero  3 , Mariano Echegorry  1 , Paola Ceriana  1 , Andrea Mora  4 , Christine Seah  5 , Alejandra Corso  1 , Roberto G Melano  5   6 , Fernando Pasteran  1
Affiliations

Emergence of KPC-31, a KPC-3 Variant Associated with Ceftazidime-Avibactam Resistance, in an Extensively Drug-Resistant ST235 Pseudomonas aeruginosa Clinical Isolate

Diego Faccone et al. Antimicrob Agents Chemother. .

Abstract

A ceftazidime-avibactam-resistant KPC-producing Pseudomonas aeruginosa strain was isolated in Argentina from a tracheal aspirate. The patient was treated with ceftazidime-avibactam in combination with other agents for 130 days. Whole-genome sequencing of P. aeruginosa identified a D179Y substitution in the Ω loop of KPC-3, corresponding to KPC-31, integrated at the chromosome. The strain belonged to the sequence type 235/O11 (ST235/O11) high-risk clone. Evaluation of carbapenemase detection assays most used by clinical laboratories failed to identify the isolate as a KPC producer.

Keywords: KPC; Pseudomonas aeruginosa; ceftazidime-avibactam resistance; extensively drug-resistant ST235.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIG 1
FIG 1
Chromosomal location of blaKPC-31 in P. aeruginosa M27432. Comparison with plasmid pGM116-005_01 from K. pneumoniae DFSAN054110 and the chromosome of P. aeruginosa AR_0357 suggests a plasmidic origin of an ~19-kb fragment containing blaKPC-31. The light blue regions between DNA sequences indicate nucleotide identity >99 by BLASTn. Arrows indicate predicted open reading frames (ORFs). Red arrows represent blaKPC gene variants.
See this image and copyright information in PMC

References

    1. Horcajada JP, Montero M, Oliver A, Sorlí L, Luque S, Gómez-Zorrilla S, Benito N, Grau S. 2019. Epidemiology and treatment of multidrug-resistant and extensively drug-resistant Pseudomonas aeruginosa infections. Clin Microbiol Rev 32:e00031-19. 10.1128/CMR.00031-19. - DOI - PMC - PubMed
    1. Pasteran F, Faccone D, Gomez S, De Bunder S, Spinelli F, Rapoport M, Petroni A, Galas M, Corso A, on behalf of the Pseudomonas aeruginosa KPC Group . 2012. Detection of an international multiresistant clone belonging to sequence type 654 involved in the dissemination of KPC-producing Pseudomonas aeruginosa in Argentina. J Antimicrob Chemother 67:1291–1293. 10.1093/jac/dks032. - DOI - PubMed
    1. Treepong P, Kos VN, Guyeux C, Blanc DS, Bertrand X, Valot B, Hocquet D. 2018. Global emergence of the widespread Pseudomonas aeruginosa ST235 clone. Clin Microbiol Infect 24:258–266. 10.1016/j.cmi.2017.06.018. - DOI - PubMed
    1. Castanheira M, Doyle TB, Smith CJ, Mendes RE, Sader HS. 2019. Combination of MexAB-OprM overexpression and mutations in efflux regulators, PBPs and chaperone proteins is responsible for ceftazidime/avibactam resistance in Pseudomonas aeruginosa clinical isolates from US hospitals. J Antimicrob Chemother 74:2588–2595. 10.1093/jac/dkz243. - DOI - PubMed
    1. Tu Y, Wang D, Zhu Y, Li J, Jiang Y, Wu W, Li X, Zhou H. 2022. Emergence of a KPC-90 variant that confers resistance to ceftazidime-avibactam in an ST463 carbapenem-resistant Pseudomonas aeruginosa strain. Microbiol Spectr 10:e01869-21. 10.1128/spectrum.01869-21. - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources